CLIA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7)

CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Beta-Thromboglobulin

Specificity

This assay has high sensitivity and excellent specificity for detection of Chemokine (C-X-C motif) ligand 7 ( CXCL7).
No significant cross-reactivity or interference between Chemokine (C-X-C motif) ligand 7 ( CXCL7) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Chemokine (C-X-C motif) ligand 7 ( CXCL7) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine (C-X-C motif) ligand 7 ( CXCL7) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 86-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C motif) ligand 7 ( CXCL7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C motif) ligand 7 ( CXCL7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine (C-X-C motif) ligand 7 ( CXCL7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 84-104% 90-102% 82-93% 95-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Clinical Hemorheology and Microcirculation Strict heart rate control attenuates prothrombotic state and platelet activity in patients with non-valvular permanent atrial fibrillation. Pubmed: 2347822
International Journal of Pharma & Bio Sciences STUDIES ON THE STORAGE OF POOLED PLATELETS IN NON DOP PVC CONTAINERS Ebscohost: Source
Journal of Affective Disorders Bioprofiling of platelets in medicated patients with depression. Pubmed:25451399
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277
Physiol Behav Differential changes in platelet reactivity induced by acute physical compared to persistent mental stress PubMed: 26192713
J Affect Disord Bioprofiling of platelets in medicated patients with depression PubMed: 25451399
Turk Kardiyol Dern Ars Markers of coagulation and fibrinolysis do not detect or predict the presence of left atrial appendage thrombus in patients with atrial fibrillation Pubmed: 32147650
JOURNAL OF FOOD BIOCHEMISTRY A novel di‐peptide Met‐Glu from collagen hydrolysates inhibits platelet aggregation and thrombus formation via regulation of Gq‐mediated signaling Pubmed: 32662128
Membranes (Basel) Endogenous Nitric Oxide-Releasing Microgel Coating Prevents Clot Formation on Oxygenator Fibers Exposed to In Vitro Blood Flow Pubmed:35054599
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA370Ra01 Active Chemokine (C-X-C motif) ligand 7 ( CXCL7) Cell culture; Activity Assays.
RPA370Ra02 Recombinant Chemokine (C-X-C motif) ligand 7 ( CXCL7) Positive Control; Immunogen; SDS-PAGE; WB.
RPA370Ra01 Recombinant Chemokine (C-X-C motif) ligand 7 ( CXCL7) Positive Control; Immunogen; SDS-PAGE; WB.
PAA370Ra01 Polyclonal Antibody to Chemokine (C-X-C motif) ligand 7 ( CXCL7) WB; IHC; ICC; IP.
PAA370Ra02 Polyclonal Antibody to Chemokine (C-X-C motif) ligand 7 ( CXCL7) WB; IHC; ICC; IP.
LAA370Ra81 FITC-Linked Polyclonal Antibody to Chemokine (C-X-C motif) ligand 7 ( CXCL7) WB; IHC; ICC; IF.
LAA370Ra71 Biotin-Linked Polyclonal Antibody to Chemokine (C-X-C motif) ligand 7 ( CXCL7) WB; IHC; ICC.
MAA370Ra22 Monoclonal Antibody to Chemokine (C-X-C motif) ligand 7 ( CXCL7) WB; IHC; ICC; IP.
SEA370Ra ELISA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA370Ra CLIA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7) Chemiluminescent immunoassay for Antigen Detection.
LMA370Ra Multiplex Assay Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA370Ra01 ELISA Kit DIY Materials for Chemokine (C-X-C motif) ligand 7 ( CXCL7) Main materials for "Do It (ELISA Kit) Yourself".