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CLIA Kit for Apolipoprotein A4 (APOA4)
ApoA-IV; ApoAIV; Apo-A4
- Product No.SCB967Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range0.21-150ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.08ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 588
For more details, please contact local distributors! US$ 840 US$ 3780 US$ 7140 US$ 58800
Specificity
This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4).
No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Apolipoprotein A4 (APOA4) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein A4 (APOA4) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 83-97 | 91 |
EDTA plasma(n=5) | 94-105 | 101 |
heparin plasma(n=5) | 78-93 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein A4 (APOA4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-96% | 98-105% | 89-97% | 90-99% |
EDTA plasma(n=5) | 91-104% | 78-102% | 80-91% | 96-103% |
heparin plasma(n=5) | 86-101% | 93-101% | 96-105% | 92-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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Magazine | Citations |
Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties PubMed: 23328279 |
Exp Ther Med. | Differential plasma proteome analysis in patients with high?altitude pulmonary edema at the acute and recovery phases Pubmed:24940404 |
EuPA Open Proteomics | Human maternal plasma proteomic changes with parturition Sciencedirect:S2212968514000506 |
BJOG | Development of concise audit standards for unplanned return to theate following gynaecological surgery at Gloucestershire Royal Hospital Doi: 10.1111 |
Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848 |
VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats Pubmed: 32880234 |
Cancer Cell Int | Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer 33602229 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPB967Hu02 | Recombinant Apolipoprotein A4 (APOA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
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PAB967Hu01 | Polyclonal Antibody to Apolipoprotein A4 (APOA4) | WB,IHC |
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KSB967Hu01 | ELISA Kit DIY Materials for Apolipoprotein A4 (APOA4) | Main materials for "Do It (ELISA Kit) Yourself". |