CLIA Kit for D-Amino Acid Oxidase (DAO)
- Product No.SCJ298Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range13.7-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.2pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 840
For more details, please contact local distributors! US$ 3780
For more details, please contact local distributors! US$ 7140
For more details, please contact local distributors! US$ 58800
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of D-Amino Acid Oxidase (DAO).
No significant cross-reactivity or interference between D-Amino Acid Oxidase (DAO) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant D-Amino Acid Oxidase (DAO) and the recovery rates were calculated by comparing the measured value to the expected amount of D-Amino Acid Oxidase (DAO) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D-Amino Acid Oxidase (DAO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|无||Differential regulation of D-amino acid oxidase (DAO) mRNA and protein expression in human post-mortem brain Dam: Jcr:3107E495-Ef68-4692-85Be-D43D9F3F84Ff|
|不确定杂志名||Age span expression of schizophrenia susceptibility genes D-amino acid oxidase (DAO) and DAO activator (DAOA) in human post-mortem brain dam:jcr:c3394762-a692-4fbd-8a37-7da14f429996|
|Frontiers in Neuroanatomy||Expression of D-Amino Acid Oxidase (DAO/DAAO) and D-Amino Acid Oxidase Activator (DAOA/G72) during Development and Aging in the Human Post-mortem Brain. pubmed:28428746|
|BMC gastroenterology||Noninvasive biomarkers of gut barrier function identify two subtypes of patients suffering from diarrhoea predominant-IBS: a case-control study Pubmed: 30400824|
|Journal of Gastroenterology||Gut microbiota dysbiosis worsens the severity of acute pancreatitis in patients and mice Pubmed: 30519748|
|Phytotherapy Research||Effect of a chamomile extract in protecting against radiation‐induced intestinal mucositis Pubmed: 30632234|
|Biological & Pharmaceutical Bulletin||Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis Pubmed: 31582652|
|Int Immunopharmacol||Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response Pubmed: 32361189|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPJ298Hu01||Recombinant D-Amino Acid Oxidase (DAO)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAJ298Hu01||Polyclonal Antibody to D-Amino Acid Oxidase (DAO)||WB; IHC; ICC; IP.|
|MAJ298Hu21||Monoclonal Antibody to D-Amino Acid Oxidase (DAO)||WB; IHC; ICC; IP.|
|SEJ298Hu||ELISA Kit for D-Amino Acid Oxidase (DAO)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCJ298Hu||CLIA Kit for D-Amino Acid Oxidase (DAO)||Chemiluminescent immunoassay for Antigen Detection.|