CLIA Kit for D-Amino Acid Oxidase (DAO)

DAMOX

Specificity

This assay has high sensitivity and excellent specificity for detection of D-Amino Acid Oxidase (DAO).
No significant cross-reactivity or interference between D-Amino Acid Oxidase (DAO) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant D-Amino Acid Oxidase (DAO) and the recovery rates were calculated by comparing the measured value to the expected amount of D-Amino Acid Oxidase (DAO) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-97 93
EDTA plasma(n=5) 90-101 93
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D-Amino Acid Oxidase (DAO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-96% 78-104% 85-94% 85-101%
EDTA plasma(n=5) 93-105% 90-104% 84-94% 85-97%
heparin plasma(n=5) 82-97% 89-98% 97-105% 80-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

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Magazine Citations
Differential regulation of D-amino acid oxidase (DAO) mRNA and protein expression in human post-mortem brain Dam: Jcr:3107E495-Ef68-4692-85Be-D43D9F3F84Ff
不确定杂志名 Age span expression of schizophrenia susceptibility genes D-amino acid oxidase (DAO) and DAO activator (DAOA) in human post-mortem brain dam:jcr:c3394762-a692-4fbd-8a37-7da14f429996
Frontiers in Neuroanatomy Expression of D-Amino Acid Oxidase (DAO/DAAO) and D-Amino Acid Oxidase Activator (DAOA/G72) during Development and Aging in the Human Post-mortem Brain. pubmed:28428746
BMC gastroenterology Noninvasive biomarkers of gut barrier function identify two subtypes of patients suffering from diarrhoea predominant-IBS: a case-control study Pubmed: 30400824
Journal of Gastroenterology Gut microbiota dysbiosis worsens the severity of acute pancreatitis in patients and mice Pubmed: 30519748
Phytotherapy Research Effect of a chamomile extract in protecting against radiation‐induced intestinal mucositis Pubmed: 30632234
Biological & Pharmaceutical Bulletin Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis Pubmed: 31582652
Int Immunopharmacol Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response Pubmed: 32361189
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