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ELISA Kit for Growth Hormone (GH)

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

Specificity

This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH).
No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Hormone (GH) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Hormone (GH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-101 90
EDTA plasma(n=5) 94-103 98
heparin plasma(n=5) 90-104 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Hormone (GH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-102% 79-90% 89-102% 92-105%
EDTA plasma(n=5) 89-96% 78-93% 87-96% 80-90%
heparin plasma(n=5) 87-96% 84-94% 87-101% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Neuroscience Letters The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats PubMed: 19765635
Cellular Reprogramming Changes in the Somatotrophic Axis in Genetically Identical Dogs PubMed: 21740269
Clinical Cancer Research The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status PubMed: 21642381
General and Comparative Endocrinology Growth hormone expression and neuroprotective activity in a quail neural retina cell line ScienceDirect: S0016648009002457
Nutrition and Metabolism Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis Nutrition&Metabolism: source
Veterinary Recprd Case Report Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism Bmj: Source
Journal of the American Animal Hospital Association GH Deficiency in a Dalmatian Puppy with Megaesophagus Pubmed: 24051259
Biotechnology and Bioprocess Engineering Functional expression of bovine growth hormone gene in Pleurotus eryngii Springer: Source
Buletin Veteriner Udayana Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali Unud:Source
Veterinary World Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows Veterinaryworld:Source
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831
Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks Pubmed:26948666
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503
Jurnal Riset Akuakultur TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA 1329
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