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ELISA Kit for Hepatocyte Growth Factor (HGF)

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

Specificity

This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF).
No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Hepatocyte Growth Factor (HGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Hepatocyte Growth Factor (HGF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-94 91
EDTA plasma(n=5) 94-101 97
heparin plasma(n=5) 93-102 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepatocyte Growth Factor (HGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 80-94% 85-103% 96-103%
EDTA plasma(n=5) 78-97% 94-103% 80-90% 83-94%
heparin plasma(n=5) 90-103% 96-103% 87-103% 91-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Reference
Gene Therapy and Molecular Biology Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart LWW: 01000
Journal of Translational Medicine Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma BioMed: 14795876
Digestive Diseases and Sciences Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure SpringerLink: k22823l138x2344x
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604
BMC cell biology BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998
Cell Journal (Yakhteh) Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis PubMed: PMC3584430
Gastroenterol Res Pract Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis PubMed: 26448742
Tumor Biology Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Cytotherapy Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury PubMed: 25863963
Biomedicine & Pharmacotherapy Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats Pubmed:27261582
Can J Physiol Pharmacol.  Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis Pubmed:27186801
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064
Changes in HGF levels after a progressive test before and after Tabata HIIT program profile:Pawel_Wolanski3
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