ELISA Kit for Interleukin 12A (IL12A)

p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 12A (IL12A).
No significant cross-reactivity or interference between Interleukin 12A (IL12A) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 12A (IL12A) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 12A (IL12A) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-97 92
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 82-104 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 12A (IL12A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-89% 88-97% 83-104% 82-94%
EDTA plasma(n=5) 82-97% 90-98% 96-104% 86-93%
heparin plasma(n=5) 80-91% 79-92% 90-97% 82-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Theriogenology Evaluation of serum concentrations of interleukin (IL)-4, IL-10, and IL-12 during pregnancy in bitches ScienceDirect: S0093691X1300037X
Anticancer Research Establishment and evaluation of a bead-based luminex assay allowing simultaneous quantification of equine IL-12 and IFN-γ. Pubmed: 23564769
Journal of Functional Foods Impact of purified oat 1-3,1-4-b-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis :
Journal of Functional Foods Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis S1756464616303528
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA059Hu01 Active Interleukin 12A (IL12A) Cell culture; Activity Assays.
RPA059Hu01 Recombinant Interleukin 12A (IL12A) Positive Control; Immunogen; SDS-PAGE; WB.
PAA059Hu01 Polyclonal Antibody to Interleukin 12A (IL12A) WB; IHC; ICC; IP.
LAA059Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 12A (IL12A) WB; IHC; ICC.
LAA059Hu81 FITC-Linked Polyclonal Antibody to Interleukin 12A (IL12A) WB; IHC; ICC; IF.
MAA059Hu21 Monoclonal Antibody to Interleukin 12A (IL12A) WB; IHC; ICC; IP.
MAA059Hu22 Monoclonal Antibody to Interleukin 12A (IL12A) WB
MAA059Hu23 Monoclonal Antibody to Interleukin 12A (IL12A) WB; IHC; ICC; IP.
MAA059Hu24 Monoclonal Antibody to Interleukin 12A (IL12A) WB
MAA059Hu26 Monoclonal Antibody to Interleukin 12A (IL12A) WB; IHC; ICC; IP.
LAA059Hu76 Biotin-Linked Monoclonal Antibody to Interleukin 12A (IL12A) WB; IHC; ICC.
SEA059Hu ELISA Kit for Interleukin 12A (IL12A) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA059Hu High Sensitive ELISA Kit for Interleukin 12A (IL12A) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA059Hu Multiplex Assay Kit for Interleukin 12A (IL12A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA059Hu01 Antibody Pair for Interleukin 12A (IL12A) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA059Hu01 ELISA Kit DIY Materials for Interleukin 12A (IL12A) Main materials for "Do It (ELISA Kit) Yourself".
IGA059Hu21 Mouse Knockin Human Interleukin 12A (IL12A) Animal Experiments; Positive Control.