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ELISA Kit for Interleukin 15 (IL15)


This assay has high sensitivity and excellent specificity for detection of Interleukin 15 (IL15).
No significant cross-reactivity or interference between Interleukin 15 (IL15) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Interleukin 15 (IL15) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 15 (IL15) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-102 97
EDTA plasma(n=5) 89-97 92
heparin plasma(n=5) 82-91 88


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 15 (IL15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 15 (IL15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 15 (IL15) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-96% 89-96% 94-105% 90-103%
EDTA plasma(n=5) 93-105% 91-99% 95-102% 90-104%
heparin plasma(n=5) 80-104% 78-94% 90-99% 79-96%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training Pubmed: 24006180
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype Pubmed:25522723
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice PubMed: 26087886
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle Dspace: Handle
Biotechnology Letters Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice s10529-017-2342-1
Journal of Controlled Release Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. pubmed:28408199
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA061Hu01 Active Interleukin 15 (IL15) Cell culture; Activity Assays.
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PAA061Hu01 Polyclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
LAA061Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC.
MAA061Hu22 Monoclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
SEA061Hu ELISA Kit for Interleukin 15 (IL15) Enzyme-linked immunosorbent assay for Antigen Detection.
IGA061Hu21 Mouse Knockin Human Interleukin 15 (IL15) Animal Experiments; Positive Control.