ELISA Kit for Interleukin 18 (IL18)
IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
- Product No.SEA064Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.5pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 6120
For more details, please contact local distributors! US$ 50400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interleukin 18 (IL18).
No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 18 (IL18) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 18 (IL18) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 18 (IL18) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|International Immunopharmacology||Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries ScienceDirect: S1567576911001068|
|Atherosclerosis||IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome ScienceDirect: S0021915009006200|
|PLoS One.||HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source|
|临床儿科杂志||中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source|
|Cell Death Dis.||MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy Pubmed:Pmc4237254|
|PLoS One.||Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434|
|J Korean Med Sci.||Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease Pubmed:25120320|
|PLOS ONE||Protective Effect of Leaf Essential Oil from PubMed: 25794175|
|Fertil Steril||Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” PubMed: 26474737|
|United States Patent Application||GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUS y2016:0138028.html|
|Front Pharmacol||Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670|
|Pharmacological reports||The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article|
|International Journal of Molecular Sciences||PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 pubmed:27973457|
|Cellular Physiology and Biochemistry||MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis. pubmed:28006785|
|Catalog No.||Related products for research use of Canis familiaris; Canine (Dog) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA064Ca01||Recombinant Interleukin 18 (IL18)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA064Ca01||Polyclonal Antibody to Interleukin 18 (IL18)||WB; IHC; ICC; IP.|
|MAA064Ca21||Monoclonal Antibody to Interleukin 18 (IL18)||WB; IHC; ICC; IP.|
|SEA064Ca||ELISA Kit for Interleukin 18 (IL18)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PGA064Ca01||Primer Pair for Interleukin 18 (IL18)||PCR|