ELISA Kit for Interleukin 1 Alpha (IL1a)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
- Product No.SEA071Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 440
For more details, please contact local distributors! US$ 1980
For more details, please contact local distributors! US$ 3740
For more details, please contact local distributors! US$ 30800
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Alpha (IL1a).
No significant cross-reactivity or interference between Interleukin 1 Alpha (IL1a) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 1 Alpha (IL1a) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 1 Alpha (IL1a) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Alpha (IL1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Alpha (IL1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 1 Alpha (IL1a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|The Veterinary Journal||Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy Pubmed: 24662024|
|Int J Clin Exp Pathol||Expression of S100 family proteins in neonatal rats with sepsis and its significance PubMed: 25973048|
|Tumor Biology||Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151|
|Parasites & Vectors||Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. pubmed:27502394|
|Cell Death Dis.||Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA071Ra02||Recombinant Interleukin 1 Alpha (IL1a)||Positive Control; Immunogen; SDS-PAGE; WB.|
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|PAA071Ra02||Polyclonal Antibody to Interleukin 1 Alpha (IL1a)||WB; IHC; ICC; IP.|
|PAA071Ra01||Polyclonal Antibody to Interleukin 1 Alpha (IL1a)||WB; IHC; ICC; IP.|
|LAA071Ra71||Biotin-Linked Polyclonal Antibody to Interleukin 1 Alpha (IL1a)||WB; IHC; ICC.|
|MAA071Ra22||Monoclonal Antibody to Interleukin 1 Alpha (IL1a)||WB; IHC; ICC; IP.|
|HEA071Ra||High Sensitive ELISA Kit for Interleukin 1 Alpha (IL1a)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEA071Ra||ELISA Kit for Interleukin 1 Alpha (IL1a)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA071Ra01||ELISA Kit DIY Materials for Interleukin 1 Alpha (IL1a)||Main materials for "Do It (ELISA Kit) Yourself".|