ELISA Kit for Immunoglobulin G1 (IgG1)

IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1

Specificity

This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G1 (IgG1).
No significant cross-reactivity or interference between Immunoglobulin G1 (IgG1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Immunoglobulin G1 (IgG1) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G1 (IgG1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 102
EDTA plasma(n=5) 78-88 83
heparin plasma(n=5) 95-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G1 (IgG1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 87-96% 91-99% 82-95%
EDTA plasma(n=5) 97-105% 79-88% 88-96% 79-103%
heparin plasma(n=5) 94-101% 81-88% 79-103% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BioMed Research International The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Hindawi:Source
Oncology Reports Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861
Cellular Immunology IgG autoantibody subclasses altered in immuno-related hemocytopenia Pubmed:25666506
Biomed Res Int The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
Biomedicine & Pharmacotherapy Icariin stimulates osteogenic differentiation of rat bone marrow stromal stem cells by increasing TAZ expression pubmed:28486190
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Pubmed: 30781605
Veterinary Microbiology Optimized GAPDH-truncated immunogen of Streptococcus equi elicits an enhanced immune response and provides effective protection in a mouse model 33647714
British Journal of Pharmacology The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPA074Hu01 Native Immunoglobulin G1 (IgG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA074Hu02 Recombinant Immunoglobulin G1 (IgG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA074Hu03 Recombinant Immunoglobulin G1 (IgG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA074Hu01 Recombinant Immunoglobulin G1 (IgG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA074Hu04 Recombinant Immunoglobulin G1 (IgG1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA074Hu01 Polyclonal Antibody to Immunoglobulin G1 (IgG1) WB; IHC; ICC; IP.
LAA074Hu71 Biotin-Linked Polyclonal Antibody to Immunoglobulin G1 (IgG1) WB; IHC; ICC.
LAA074Hu81 FITC-Linked Polyclonal Antibody to Immunoglobulin G1 (IgG1) WB; IHC; ICC; IF.
MAA074Hu23 Monoclonal Antibody to Immunoglobulin G1 (IgG1) WB; IHC; ICC; IP.
MAA074Hu21 Monoclonal Antibody to Immunoglobulin G1 (IgG1) WB; IHC; ICC; IP.
MAA074Hu22 Monoclonal Antibody to Immunoglobulin G1 (IgG1) WB; IHC; ICC; IP.
MAA074Hu24 Monoclonal Antibody to Immunoglobulin G1 (IgG1) WB; IHC; ICC; IP.
SEA074Hu ELISA Kit for Immunoglobulin G1 (IgG1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA074Hu Multiplex Assay Kit for Immunoglobulin G1 (IgG1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA074Hu01 ELISA Kit DIY Materials for Immunoglobulin G1 (IgG1) Main materials for "Do It (ELISA Kit) Yourself".