ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)
CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE
- Product No.SEA087Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.4pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
Ensure in stock; if not, free redo!
For negotiated price and more details, please contact local distributors! US$ 630
For negotiated price and more details, please contact local distributors! US$ 2835
For negotiated price and more details, please contact local distributors! US$ 5355
For negotiated price and more details, please contact local distributors! US$ 44100
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 1 (MCP1).
No significant cross-reactivity or interference between Monocyte Chemotactic Protein 1 (MCP1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Monocyte Chemotactic Protein 1 (MCP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Monocyte Chemotactic Protein 1 (MCP1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Monocyte Chemotactic Protein 1 (MCP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Cytokine||Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression. Pubmed: 23434274|
|Rheumatology International||Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186|
|Food & Function||Effects of Hyriopsis cumingii polysaccharides on angiogenesis, macrophage chemotaxis, proliferation and phagocytosis Pubmed:25620195|
|Nucleic Acids Res.||MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells Pubmed:Pmc4176337|
|Journal of Trace Elements in Medicine and Biology||Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats Pubmed:25194956|
|Int J Clin Exp Pathol||Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis PubMed: 26191125|
|Mediators of Inflammation||Effects of Intravenous Injection of PubMed: 26063970|
|Diabetes||Protein Inhibitor of Activated STAT 1 (PIAS1) Protects Against Obesity-Induced Insulin Resistance by Inhibiting Inflammation Cascade in Adipose Tissue PubMed: 26324179|
|Journal of Histochmistry and Cytochmistry||Epigallocatechin-3-Gallate Attenuates Unilateral Ureteral Obstruction-Induced Renal Interstitial Fibrosis in Mice PubMed: 25549657|
|Cell Physiol Biochem||MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1 PubMed: 26159489|
|Exp Biol Med (Maywood)||Renoprotective effects of angiotensin receptor blocker and stem cells in acute kidney injury: Involvement of inflammatory and apoptotic markers PubMed: 25825359|
|Parasitol Res||Trypanosoma cruzi infection and benznidazole therapy independently stimulate oxidative status and structural pathological remodeling of the liver tissue in mice PubMed: 25912691|
|Invest Ophthalmol Vis Sci||Simultaneous Increase in Multiple Proinflammatory Cytokines in the Aqueous Humor in Neovascular Glaucoma With and Without Intravitreal Bevacizumab Injection PubMed: 26030108|
|PLoS One||Postoperative changes in aqueous monocyte chemotactic protein-1 levels and bleb morphology after trabeculectomy vs. Ex-PRESS shunt surgery PubMed: 26427058|
|Veterinary Parasitology||Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140|
|J Pharm Pharmacol||Modulation of oxidative and inflammatory cardiac response by nonselective 1‐and 3‐cyclooxygenase inhibitor and benznidazole in mice PubMed: 26105112|
|Микроэлементы в медицине||НАРУШЕНИЕ БАЛАНСА ХРОМА И ВАНАДИЯ В ЖИРОВОЙ ТКАНИ КАК ВОЗМОЖНЫЙ МЕХАНИЗМ ОЖИРЕНИЕ-АССОЦИИРОВАННОЙ ИНСУЛИНОРЕЗИСТЕНТНОСТИ Microelements_Of_Midicine: 2015_2|
|Front Pharmacol||The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling Pathway PubMed: 26696888|
|PLoS One||Effects of PB-EPCs on Homing Ability of Rabbit BMSCs via Endogenous SDF-1 and MCP-1. PubMed: 26660527|
|Scientific Reports||The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra Pubmed:26912204|
|Neural Plasticity||Fractalkine Attenuates Microglial Cell Activation Induced by Prenatal Stress Pubmed:27239349|
|Technologia Alimentaria||Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats abstract:10|
|International Immunopharmacology||Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602|
|J Neurochem.||Anti‐inflammatory properties of tianeptine on lipopolysaccharide‐induced changes in microglial cells involve toll‐like receptor‐related pathways Pubmed:26640965|
|Oxidative Medicine and Cellular Longevity||Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. pubmed:27656261|
|Comparative Clinical Pathology||Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets article:10.1007|
|clinical chemistry and laboratory medicine||The serum concentrations of leptin and MCP-1 independently predict low back pain duration. pubmed:28076310|
|Journal of Molecular Cell Biology||oxLDL antibody inhibits MCP‐1 release in monocytes/macrophages by regulating Ca2+/K+ channel flow pubmed:27995732|
|Parasitology.||Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice. pubmed:28134069|