ELISA Kit for Interleukin 12 (IL12)

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 12 (IL12).
No significant cross-reactivity or interference between Interleukin 12 (IL12) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 12 (IL12) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 12 (IL12) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-101 82
EDTA plasma(n=5) 79-101 90
heparin plasma(n=5) 79-94 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 12 (IL12) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 94-101% 86-96% 95-103%
EDTA plasma(n=5) 79-99% 90-98% 79-98% 95-104%
heparin plasma(n=5) 79-90% 88-103% 87-94% 78-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:
Cancer Science A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme … Scholarena:Source
International Immunopharmacology Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment Pubmed:30015235
Oncology Letters Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro Pubmed:30127867
Neuroscience The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat joints Pubmed: 30248434
Cancer Biomarkers Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in … Pubmed: 30223387
International Journal of Clinical and Experimental Medicine The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice
ВЛИЯНИЕ ДНК МЕТИЛТРАНСФЕРАЗЫ 3А И ГИСТОНДЕАЦЕТИЛАЗЫ НА СОДЕРЖАНИЕ В МОНОНУКЛЕАРНЫХ КЛЕТКАХ ЦЕЛЬНОЙ КРОВИ …
Experimental and Therapeutic Medicine RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery
Chemosphere Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus
Infection and Drug Resistance Disseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report. Pubmed: 31632104
Biomed Pharmacother Suppression of Fpr2 expression protects against endotoxin-induced acute lung injury by interacting with Nrf2-regulated TAK1 activation Pubmed: 32106380
Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signaling Pubmed: 31993149
J Vet Med Sci Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cows Pubmed: 33071254
Batı Karadeniz Tıp Dergisi Vasküler Demans Modelinde Çevresel Zenginleştirmenin Depresyon Benzeri Davranış, Kortikal ve Hipokampal BDNF ve IL-1β Seviyeleri Üzerine Etkisi
Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra
Life Sci BBG enhances OLT1177-induced NLRP3 inflammasome inactivation by targeting P2X7R/NLRP3 and MyD88/NF-¦ÊB signaling in DSS-induced colitis in rats 33548287
IUBMB Life HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1 34117711
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
SEA111Ga ELISA Kit for Interleukin 12 (IL12) Enzyme-linked immunosorbent assay for Antigen Detection.