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ELISA Kit for Placenta Growth Factor (PLGF)

PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Placenta Growth Factor (PLGF).
No significant cross-reactivity or interference between Placenta Growth Factor (PLGF) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Placenta Growth Factor (PLGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Placenta Growth Factor (PLGF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-103 98
EDTA plasma(n=5) 94-103 99
heparin plasma(n=5) 83-97 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Placenta Growth Factor (PLGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-103% 81-96% 97-105% 83-91%
EDTA plasma(n=5) 95-104% 88-96% 83-94% 79-92%
heparin plasma(n=5) 85-103% 90-98% 82-98% 91-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Reference
Microvascular Research Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
?eLife Photoreceptor avascular privilege is shielded by soluble VEGF receptor-1 PubMed: PMC3687373
International Journal of Nanomedicine Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis Pubmed:27042064
The Journal of Maternal-Fetal & Neonatal Medicine Serum angiogenic profile in abnormal placentation Pubmed:26863111
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study Pubmed:27143518
Basic & Clinical Pharmacology & Toxicology Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway doi:10.1111
Life Sci. Placenta growth factor contributes to cell apoptosis and epithelial-to-mesenchymal transition in the hyperoxia-induced acute lung injury Pubmed:27211521
Int Immunopharmacol Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway Pubmed:27280587
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA114Hu01 Recombinant Placenta Growth Factor (PLGF) Positive Control; Immunogen; SDS-PAGE; WB.
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MAA114Hu22 Monoclonal Antibody to Placenta Growth Factor (PLGF) WB; IHC; ICC; IP.
SEA114Hu ELISA Kit for Placenta Growth Factor (PLGF) Enzyme-linked immunosorbent assay for Antigen Detection.