ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)
CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
- Product No.SEA122Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 438
For more details, please contact local distributors! US$ 1971
For more details, please contact local distributors! US$ 3723
For more details, please contact local distributors! US$ 30660
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1).
No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Stromal Cell Derived Factor 1 (SDF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Stromal Cell Derived Factor 1 (SDF1) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||83-92||88|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stromal Cell Derived Factor 1 (SDF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||85-96%||80-103%||97-105%||80-90%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Critical Care||Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD) Goescholar: source|
|22||The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels ScienceDirect: S0142961209012654|
|22||The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms ScienceDirect: S0142961211011422|
|Phytomedicine||Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model ScienceDirect: S0944711310003260|
|Graefe's Archive for Clinical and Experimental Ophthalmology||Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells PubMed: 21655971|
|Molecular Therapy||Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia PubMed: 21224834|
|Oncogene||Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069|
|International Journal of Nephrology||Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis PubMed: 22792461|
|Rheumatology International||Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186|
|Experimental Eye Research||Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells Pubmed: 24780853|
|Atherosclerosis||The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial Pubmed:24530788|
|International Journal of Cardiology||Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men Pubmed:24286591|
|Tissue Engineering Part A||Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat Pubmed:24814095|
|Pharmacol Rep.?||Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression Pubmed:25150002|
|Journal of Stroke and Cerebrovascular Diseases||Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients Pubmed:25444027|
|Pharmacological Reports||The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders Pubmed:25560580|
|In Vitro Cellular & Developmental Biology - Animal||Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells Pubmed:25636237|
|Pharmacological Reports||Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder PubMed: 26481549|
|Biochem Biophys Res Commun||Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats PubMed: 26392311|
|Am J Transl Res||Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666|
|Medicine (Baltimore)||A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion PubMed: 26632727|
|Pharmacological Reports||A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity Pubmed:27208878|
|Frontiers in Psychiatry||Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. pubmed:28149283|
|European Journal of Applied Physiology||High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia. pubmed:27761657|
|Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease||AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs pubmed:29233725|
|International Journal of Clinical and Experimental Pathology||Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424|
|BioMed Research International.||c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer pubmed:28573141|
|Scientific Reports||Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms. pubmed:28842629|
|Biomaterials||Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study. pubmed:29017079|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
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