ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)
TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
- Product No.SEA124Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.0pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 1 (TGFb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 1 (TGFb1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 1 (TGFb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Cardiovascular Research||Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway OxfordJournals: source|
|Immunopharmacology and Inflammation||Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid ScienceDirect: S0188440910001049|
|Biochemical and Biophysical Research Communications||Chronic angiotensin-(1–7) administration improves vascular remodeling after angioplasty through the regulation of the TGF-β/Smad signaling pathway in rabbits ScienceDirect: S0006291X09017021|
|European Cells and Materials||Incorporating pTGF-β1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: efficient, sustained gene delivery to stem cells for chondrogenic differentiation. Pubmed: 22314694|
|Journal of Animal and Veterinary Advances||A Soluble Antigen-Specific Factor from CD4+CD25+ T Cells of OVA Tolerant Mice Inducing OVA-Specific Peripheral Tolerance Independently in vivo Medwell: 2011642650|
|revue de medecine veterinaire||Increased circulating concentrations of PDGF-BB and TGF-β1 in canine generalised demodicosis Revmedvet: Source|
|Biomedical and Environmental Sciences||The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats Besjournal: Source|
|The Open Respiratory Medicine Journal||Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240|
|PLoS ONE||A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source|
|J Physiol Pharmacol.||Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet Pubmed: 23568967|
|international Journal of food and nutritional sciences||EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source|
|Journal of ethnopharmacology||Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. Pubmed: 24211397|
|Int J Clin Exp Pathol||Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of esenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration Ijcep:Source|
|Am J Physiol Regul Integr Comp Physiol.||Prolactin anterior pituitary expression and circulating levels are reduced in obese and diabetic rats: Role of TGFβ and TNFα Pubmed:25715833|
|Exp Ther Med.||PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis Pubmed:Pmc4079423|
|International Journal of Molecular Medicine||Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats Pubmed:25524396|
|Indian J Pharmacol||Effect of coenzyme Q10 alone and its combination with metformin on streptozotocin-nicotinamide-induced diabetic nephropathy in rats Pubmed:25538335|
|Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA)||Ameliorative potential of omega 3 fatty acids and HMG-CoA reductase inhibitors on experimentally-induced non-alcoholic steatohepatitis Pubmed:25541279|
|Anadolu Kardiyol Derg.||A novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes. Pubmed:25592103|
|Journal of Drug||Targeting Apoptosis, Advanced Glycation End Products and Transforming Growth Factor-β1 by Biguanides and Resveratrol to Ameliorate Experimentally-induced Diabetic Nephropathy Pdf: Drugs|
|Mol Med Rep||NIM811 downregulates transforming growth factor PubMed: 26573209|
|The Tohoku Journal of Experimental Medicine||Salvia Miltiorrhiza Bge.f.alba Ameliorates the Progression of Monocrotaline-Induced Pulmonary Hypertension by Protecting Endothelial Injury in Rats PubMed: 26074502|
|Oncol Rep||Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury PubMed: 26717975|
|J Surg Res||Epigallocatechin-3-gallate reduces tubular cell apoptosis in mice with ureteral obstruction PubMed: 25913488|
|Histol Histopathol||Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells PubMed: 26254457|
|Clin Exp Pharmacol Physiol||Silymarin attenuates paraquat-induced lung injury via Nrf2-mediated pathway in vivo and in vitro PubMed: 26173462|
|Immunologic Research||Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE6 and is crucial for liver transplant tolerance PubMed: 25982596|
|Journal of Neuroscience||MSX3 Switches Microglia Polarization and Protects from Inflammation-Induced Demyelination PubMed: 25904788|
|J Reprod Immunol||The immunomodulating effect of seminal plasma on T cells PubMed: 25799173|
|Nutr Cancer||Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-4 Infected Patients in Egypt PubMed: 26492130|
|Int J Clin Exp Med||Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … PubMed: 26309506|
|Int J Clin Exp Pathol||Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration. PubMed: 25972996|
|International Immunopharmacology||Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways Pubmed:27179304|
|Deletion Polymorphism and Chronic Obstructive Pulmonary Disease||ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970|
|Tissue and Cell||Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects Pubmed:27233913|
|Cancer Medicine & Anti Cancer Drugs||Ameliorative Potential of Tamoxifen/Thymoquinone Combination in Patients with Breast Cancer: A Biochemical and Immunohistochemical Study open-access:1000102|
|Sleep Sciences||Th17 Cell Related Cytokine Profiles in Narcolepsy and Other Types of Excessive Daytime Sleepiness article:15|
|Tissue Cell.||Ameliorative potential of fluoxetine/raloxifene combination on experimentally induced breast cancer Pubmed:26881735|
|International Journal of Molecular Sciences||TGF-β and Physiological Root Resorption of Deciduous Teeth. pubmed:28035998|
|BMC Veterinary Research||Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600|
|immunology letters||Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila. pubmed:28366526|
|immunology letters||Gemcitabine treatment enhanced the anti-tumor effect of cytokine induced killer cells by depletion of CD4+CD25bri regulatory T cells. pubmed:27867030|
|Journal of Cardiovascular Electrophysiology||Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. pubmed:28127817|
|Molecular Medicine Reports||The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. pubmed:27484042|
|International Immunopharmacology||Amelioration of bleomycin-induced lung fibrosis in rats by valproic acid and butyrate: Role of nuclear factor kappa-B, proinflammatory cytokines and oxidative stress pubmed:27526269|
|Cellular Physiology and Biochemistry||Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753|
|Canadian Journal of Physiology and Pharmacology||The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats pubmed:28177680|
|PLoS One.||Differential frequency of CD8+ T cell subsets in multiple sclerosis patients with various clinical patterns pubmed:27467597|
|Therapeutics and Clinical Risk Management||Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. pubmed:27540296|
|Journal of Cancer Research and Treatment||Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines jcrt:5|
|Sci Rep||Follistatin like-1 aggravates silica-induced mouse lung injury PMC5428474|
|Ren Fail.||Effect of concomitant administration of coenzyme Q10 with sitagliptin on experimentally induced diabetic nephropathy in rats. pubmed:27841100|
|Basic Clin Pharmacol Toxicol.||Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440|
|Acta Vet Hung.||Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. pubmed:28244338|
|Environmental Toxicology and Pharmacology||Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study pubmed:28192751|
|PeerJ||Platelet-rich concentrate in serum free medium enhances osteogenic differentiation of bone marrow-derived human mesenchymal stromal cells pubmed:27651984|