• Promotion
ELISA Kit for Carcinoembryonic Antigen (CEA)

CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen

Specificity

This assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen (CEA).
No significant cross-reactivity or interference between Carcinoembryonic Antigen (CEA) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Carcinoembryonic Antigen (CEA) and the recovery rates were calculated by comparing the measured value to the expected amount of Carcinoembryonic Antigen (CEA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-102 99
EDTA plasma(n=5) 94-105 101
heparin plasma(n=5) 80-102 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carcinoembryonic Antigen (CEA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carcinoembryonic Antigen (CEA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carcinoembryonic Antigen (CEA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 92-101% 86-94% 95-105%
EDTA plasma(n=5) 78-101% 84-102% 86-94% 78-99%
heparin plasma(n=5) 98-105% 84-102% 95-103% 96-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Reference
BioFactors Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761
Life Sciences Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700
Iranian journal of basic medical sciences Saffron Aqueous Extract Inhibits the Chemically-induced Gastric Cancer Progression in the Wistar Albino Rat PubMed: PMC3637902
Biosensors and Bioelectronics Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip Pubmed: 23672876
Asian Pac J Cancer Prev. Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529
Journal of Analytical Atomic Spectrometry Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source
Breast Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator PubMed: 26446295
The Journal of Toxicological Sciences Gene expression analysis in the lung of the rasH2 transgenic mouse at week 4 prior to induction of malignant tumor formation by urethane and N-methylolacrylamide PubMed: 26558449
PLoS One Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study Pubmed:26934615
Journal of Applied Pharmaceutical Science Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf
Analytical chemistry Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells pubmed:27506255
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA150Hu61 Active Carcinoembryonic Antigen (CEA) Cell culture; Activity Assays.
EPA150Hu61 Eukaryotic Carcinoembryonic Antigen (CEA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA150Hu01 Recombinant Carcinoembryonic Antigen (CEA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA150Hu04 Recombinant Carcinoembryonic Antigen (CEA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA150Hu03 Recombinant Carcinoembryonic Antigen (CEA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA150Hu02 Recombinant Carcinoembryonic Antigen (CEA) Positive Control; Immunogen; SDS-PAGE; WB.
PAA150Hu04 Polyclonal Antibody to Carcinoembryonic Antigen (CEA) WB; IHC; ICC; IP.
PAA150Hu03 Polyclonal Antibody to Carcinoembryonic Antigen (CEA) WB; IHC; ICC; IP.
PAA150Hu01 Polyclonal Antibody to Carcinoembryonic Antigen (CEA) WB; IHC; ICC; IP.
PAA150Hu02 Polyclonal Antibody to Carcinoembryonic Antigen (CEA) WB; IHC; ICC; IP.
LAA150Hu71 Biotin-Linked Polyclonal Antibody to Carcinoembryonic Antigen (CEA) WB; IHC; ICC.
MAA150Hu22 Monoclonal Antibody to Carcinoembryonic Antigen (CEA) WB; IHC; ICC; IP.
SEA150Hu ELISA Kit for Carcinoembryonic Antigen (CEA) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA150Hu01 ELISA Kit DIY Materials for Carcinoembryonic Antigen (CEA) Main materials for "Do It (ELISA Kit) Yourself".