ELISA Kit for Carcinoembryonic Antigen (CEA)
CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen
- Product No.SEA150Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range19.6-1,250pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 7.5pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 419
For more details, please contact local distributors! US$ 1886
For more details, please contact local distributors! US$ 3562
For more details, please contact local distributors! US$ 29330
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen (CEA).
No significant cross-reactivity or interference between Carcinoembryonic Antigen (CEA) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Carcinoembryonic Antigen (CEA) and the recovery rates were calculated by comparing the measured value to the expected amount of Carcinoembryonic Antigen (CEA) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carcinoembryonic Antigen (CEA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carcinoembryonic Antigen (CEA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carcinoembryonic Antigen (CEA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|BioFactors||Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761|
|Life Sciences||Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700|
|Iranian journal of basic medical sciences||Saffron Aqueous Extract Inhibits the Chemically-induced Gastric Cancer Progression in the Wistar Albino Rat PubMed: PMC3637902|
|Biosensors and Bioelectronics||Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip Pubmed: 23672876|
|Asian Pac J Cancer Prev.||Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529|
|Journal of Analytical Atomic Spectrometry||Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source|
|Breast||Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator PubMed: 26446295|
|The Journal of Toxicological Sciences||Gene expression analysis in the lung of the rasH2 transgenic mouse at week 4 prior to induction of malignant tumor formation by urethane and N-methylolacrylamide PubMed: 26558449|
|PLoS One||Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study Pubmed:26934615|
|Journal of Applied Pharmaceutical Science||Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf|
|Analytical chemistry||Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells pubmed:27506255|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|APA150Hu61||Active Carcinoembryonic Antigen (CEA)||Cell culture; Activity Assays.|
|EPA150Hu61||Eukaryotic Carcinoembryonic Antigen (CEA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA150Hu01||Recombinant Carcinoembryonic Antigen (CEA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA150Hu04||Recombinant Carcinoembryonic Antigen (CEA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA150Hu03||Recombinant Carcinoembryonic Antigen (CEA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA150Hu02||Recombinant Carcinoembryonic Antigen (CEA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA150Hu04||Polyclonal Antibody to Carcinoembryonic Antigen (CEA)||WB; IHC; ICC; IP.|
|PAA150Hu03||Polyclonal Antibody to Carcinoembryonic Antigen (CEA)||WB; IHC; ICC; IP.|
|PAA150Hu01||Polyclonal Antibody to Carcinoembryonic Antigen (CEA)||WB; IHC; ICC; IP.|
|PAA150Hu02||Polyclonal Antibody to Carcinoembryonic Antigen (CEA)||WB; IHC; ICC; IP.|
|LAA150Hu71||Biotin-Linked Polyclonal Antibody to Carcinoembryonic Antigen (CEA)||WB; IHC; ICC.|
|MAA150Hu22||Monoclonal Antibody to Carcinoembryonic Antigen (CEA)||WB; IHC; ICC; IP.|
|SEA150Hu||ELISA Kit for Carcinoembryonic Antigen (CEA)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA150Hu01||ELISA Kit DIY Materials for Carcinoembryonic Antigen (CEA)||Main materials for "Do It (ELISA Kit) Yourself".|