ELISA Kit for Alpha-Fetoprotein (aFP)
A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
- Product No.SEA153Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.127ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP).
No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Alpha-Fetoprotein (aFP) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-Fetoprotein (aFP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fetoprotein (aFP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fetoprotein (aFP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-Fetoprotein (aFP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|BioFactors||Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761|
|Food and Nutrition Sciences||Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. Scirp: 5684|
|Diabetes Research and Clinical Practice||Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats ScienceDirect: S0168822711000520|
|Life Sciences||Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700|
|Annals of Hepatology||Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. Hepatology: source|
|Biomedicine & Aging Pathology||Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats ScienceDirect: S2210522013000348|
|European journal of pharmacology||Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413|
|Asian Pac J Cancer Prev.||Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529|
|Journal of Analytical Atomic Spectrometry||Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source|
|PLOS ONE||Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma Pubmed:24927126|
|BMC Med Imaging||Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma PubMed: 25981587|
|Tumour Biol||Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma PubMed: 26643893|
|Acta Veterinaria Brno||The concentrations of selected blood serum proteins in calves during the first three months of life 85:33|
|Hepatobiliary Pancreat Dis Int||Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml|
|Journal of Applied Pharmaceutical Science||Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA153Ra01||Recombinant Alpha-Fetoprotein (aFP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA153Ra01||Polyclonal Antibody to Alpha-Fetoprotein (aFP)||WB; IHC; ICC; IP.|
|LAA153Ra81||FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (aFP)||WB; IHC; ICC; IF.|
|LAA153Ra71||Biotin-Linked Polyclonal Antibody to Alpha-Fetoprotein (aFP)||WB; IHC; ICC.|
|MAA153Ra21||Monoclonal Antibody to Alpha-Fetoprotein (aFP)||WB; IHC; ICC; IP.|
|SEA153Ra||ELISA Kit for Alpha-Fetoprotein (aFP)||Enzyme-linked immunosorbent assay for Antigen Detection.|