ELISA Kit for Anti-Mullerian Hormone (AMH)
MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance
- Product No.SEA228Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.4pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH).
No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Anti-Mullerian Hormone (AMH) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Mullerian Hormone (AMH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Mullerian Hormone (AMH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Zhejiang University SCIENCE B||Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. PubMed: 23125082|
|In Vivo.(Athens,Greece)||Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF Pubmed: 24292596|
|Gynecological Endocrinology||The effects of isotretinoin on the ovarian reserve of females with acne Informahealthcare: Source|
|Fertility and Sterility||Increased expression of anti mullerian hormone and its receptor in endometriosis Ingentaconnect: Source|
|Reproductive BioMedicine Online||Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure Rbmojournal: Source|
|Reproductive Biology and Endocrinology||The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. Pubmed: 24341292|
|Biomed Res Int.||Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats Pubmed:Pmc4053297|
|Fertil Steril.||Increased expression of antimüllerian hormone and its receptor in endometriosis Pubmed:24613539|
|The Journal of Maternal-Fetal & Neonatal Medicine||Maternal serum anti-Müllerian hormone at 11-13 weeks' gestation in the prediction of preeclampsia Pubmed:24953352|
|J Gynecol Oncol.||Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model Pubmed:25142624|
|Reproductive BioMedicine Online||The effect of childhood cancer therapy on ovarian reserve and pubertal development Pubmed:25498597|
|Reproductive Sciences||Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201|
|Yonsei Medical Journal||Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines PubMed: 26632380|
|oncology research||Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940|
|Reprod Biomed Online||Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats PubMed: 26206282|
|PLoS One||Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis PubMed: 26465611|
|Toxicol Lett||Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood PubMed: 26657179|
|Journal of Assisted Reproduction and Genetics||Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage? Pubmed:27255570|
|Anticancer Res.||Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer Pubmed:26976997|
|Chemosphere.||Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155|
|Theriogenology||Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. pubmed:28043339|
|environmental pollution||Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061|
|Journal of Assisted Reproduction and Genetics||Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. pubmed:28074436|
|Journal Pakistan Medical Association||Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice pubmed:27654725|
|Advance Medical Associates||Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? pubmed:27162065|
|Yonsei Med J Volume||Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. pubmed:26632380|
|Journal of Endocrinological Investigation||The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF. pubmed:27465668|
|Catalog No.||Related products for research use of Chicken (Gallus) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA228Ga01||Recombinant Anti-Mullerian Hormone (AMH)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA228Ga01||Polyclonal Antibody to Anti-Mullerian Hormone (AMH)||WB; IHC; ICC; IP.|
|SEA228Ga||ELISA Kit for Anti-Mullerian Hormone (AMH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CGA228Ga01||cDNA of Anti-Mullerian Hormone (AMH)||PCR; Protein Expression; etc.|