ELISA Kit for Anti-Mullerian Hormone (AMH)

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Specificity

This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH).
No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-Mullerian Hormone (AMH) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Mullerian Hormone (AMH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-96 90
EDTA plasma(n=5) 93-105 98
heparin plasma(n=5) 82-89 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Mullerian Hormone (AMH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 95-103% 83-93% 91-105%
EDTA plasma(n=5) 84-92% 83-102% 82-98% 78-88%
heparin plasma(n=5) 98-105% 96-105% 88-101% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Reference
Journal of Zhejiang University SCIENCE B Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. PubMed: 23125082
In Vivo.(Athens,Greece) Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF Pubmed: 24292596
Gynecological Endocrinology The effects of isotretinoin on the ovarian reserve of females with acne Informahealthcare: Source
Fertility and Sterility Increased expression of anti mullerian hormone and its receptor in endometriosis Ingentaconnect: Source
Reproductive BioMedicine Online Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure Rbmojournal: Source
Reproductive Biology and Endocrinology The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. Pubmed: 24341292
Biomed Res Int. Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats Pubmed:Pmc4053297
Fertil Steril. Increased expression of antimüllerian hormone and its receptor in endometriosis Pubmed:24613539
The Journal of Maternal-Fetal &amp; Neonatal Medicine Maternal serum anti-Müllerian hormone at 11-13 weeks&#039; gestation in the prediction of preeclampsia Pubmed:24953352
J Gynecol Oncol. Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model Pubmed:25142624
Reproductive BioMedicine Online The effect of childhood cancer therapy on ovarian reserve and pubertal development Pubmed:25498597
Reproductive Sciences Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201
Yonsei Medical Journal Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines PubMed: 26632380
oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940
Reprod Biomed Online Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats PubMed: 26206282
PLoS One Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis PubMed: 26465611
Toxicol Lett Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood PubMed: 26657179
Journal of Assisted Reproduction and Genetics Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage? Pubmed:27255570
Anticancer Res. Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer Pubmed:26976997
Chemosphere. Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155
Theriogenology Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. pubmed:28043339
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
Journal of Assisted Reproduction and Genetics Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. pubmed:28074436
Journal Pakistan Medical Association Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice pubmed:27654725
Advance Medical Associates Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? pubmed:27162065
Yonsei Med J Volume Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. pubmed:26632380
Journal of Endocrinological Investigation The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF. pubmed:27465668
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