ELISA Kit for Histone H3 (H3)


This assay has high sensitivity and excellent specificity for detection of Histone H3 (H3).
No significant cross-reactivity or interference between Histone H3 (H3) and analogues was observed.


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone H3 (H3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone H3 (H3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Biomed Res Int A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890
Catalog No. Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA285Mi01 Recombinant Histone H3 (H3) Positive Control; Immunogen; SDS-PAGE; WB.
CAA285Mi01 Anti-Histone H3 (H3) Polyclonal Antibody Loading Control of WB.
PAA285Mi01 Polyclonal Antibody to Histone H3 (H3) WB; IHC; ICC; IP.
SEA285Mi ELISA Kit for Histone H3 (H3) Enzyme-linked immunosorbent assay for Antigen Detection.