ELISA Kit for Lactoperoxidase (LPO)

SPO; Salivary peroxidase

Specificity

This assay has high sensitivity and excellent specificity for detection of Lactoperoxidase (LPO).
No significant cross-reactivity or interference between Lactoperoxidase (LPO) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Med Sci Monit.? The assessment of sIgA, histatin-5, and lactoperoxidase levels in saliva of adolescents with dental caries Pubmed:Pmc4087079
Journal of clinical periodontology Association of salivary peroxidase activity and concentration with periodontal health–a validity study pubmed:28555944
Oncology Letters Mechanism of anticancer action of novel berenil complex of platinum (II) combined with anti-MUC1 in MCF-7 breast cancer cells Pubmed:29434943
Validitätsstudie zur Assoziation von Speichelperoxidase-Aktivität und-Konzentration mit der parodontalen Gesundheit
BRAIN RESEARCH Cerebrospinal fluid lactoperoxidase level is enhanced in idiopathic Parkinson's disease, and correlates with levodopa equivalent daily dose 33676939
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPA296Bo01 Recombinant Lactoperoxidase (LPO) Positive Control; Immunogen; SDS-PAGE; WB.
PAA296Bo01 Polyclonal Antibody to Lactoperoxidase (LPO) WB; IHC; ICC; IP.
MAA296Bo21 Monoclonal Antibody to Lactoperoxidase (LPO) WB; IHC; ICC; IP.
SEA296Bo ELISA Kit for Lactoperoxidase (LPO) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA296Bo CLIA Kit for Lactoperoxidase (LPO) Chemiluminescent immunoassay for Antigen Detection.
LMA296Bo Multiplex Assay Kit for Lactoperoxidase (LPO) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.