ELISA Kit for Collagen Type I Alpha 1 (COL1a1)
COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
- Product No.SEA350Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.57ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1).
No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Collagen Type I Alpha 1 (COL1a1) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type I Alpha 1 (COL1a1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type I Alpha 1 (COL1a1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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|Journal of Biomedical Materials||Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study Wiley: source|
|European Archives of Oto-Rhino-Laryngology||Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847|
|J Voice.||Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429|
|Arch Oral Biol.||Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Pubmed: 24370188|
|Laryngoscope.||Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625|
|UNIVERSA MEDICINA||Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture Univmed:Source|
|International Journal of Neuropsychopharmacology||The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. pubmed:27477355|
|National Natural Scientific||Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396|
|Nephron.||Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor pubmed:28768285|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA350Mu01||Recombinant Collagen Type I Alpha 1 (COL1a1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA350Mu02||Recombinant Collagen Type I Alpha 1 (COL1a1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA350Mu01||Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)||WB; IHC; ICC; IP.|
|PAA350Mu02||Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)||WB; IHC; ICC; IP.|
|LAA350Mu71||Biotin-Linked Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)||WB; IHC; ICC.|
|SEA350Mu||ELISA Kit for Collagen Type I Alpha 1 (COL1a1)||Enzyme-linked immunosorbent assay for Antigen Detection.|