ELISA Kit for Lipoprotein lipase (LPL)
LIPD; Lipase, Lipoprotein
- Product No.SEA386Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.625-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.226ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Lipoprotein lipase (LPL).
No significant cross-reactivity or interference between Lipoprotein lipase (LPL) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Lipoprotein lipase (LPL) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipoprotein lipase (LPL) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipoprotein lipase (LPL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipoprotein lipase (LPL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipoprotein lipase (LPL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Diabetes||Severity of Diabetes Governs Vascular Lipoprotein Lipase by Affecting Enzyme Dimerization and Disassembly PubMed: 21646389|
|The Korean Journal of Internal Medicine||Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation Pubmed:27243230|
|The Journal of Lipid Research||ReCavia (Guinea pig )lation of Plasma Lipid Homeostasis by Hepatic Lipoprotein Lipase in Adult Mice Pubmed:27234787|
|Journal of Functional Foods||Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet 10.1016/j.jff.2017.07.020|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA386Hu02||Recombinant Lipoprotein lipase (LPL)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA386Hu01||Recombinant Lipoprotein lipase (LPL)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA386Hu02||Polyclonal Antibody to Lipoprotein lipase (LPL)||WB; IHC; ICC; IP.|
|PAA386Hu01||Polyclonal Antibody to Lipoprotein lipase (LPL)||WB; IHC; ICC; IP.|
|MAA386Hu22||Monoclonal Antibody to Lipoprotein lipase (LPL)||WB; IHC; ICC; IP.|
|SEA386Hu||ELISA Kit for Lipoprotein lipase (LPL)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CGA386Hu02||cDNA of Lipoprotein lipase (LPL)||PCR; Protein Expression; etc.|