ELISA Kit for Caspase 7 (CASP7)

CMH1; ICE-LAP3; MCH3; Caspase 7 Apoptosis-Related Cysteine Peptidase; Cysteine Aspartate Protease 7; Cysteinyl Aspartate Specific Proteinases 7

Specificity

This assay has high sensitivity and excellent specificity for detection of Caspase 7 (CASP7).
No significant cross-reactivity or interference between Caspase 7 (CASP7) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 7 (CASP7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 7 (CASP7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
International Journal of Biological Sciences Protective Effects of Morus alba Leaves Extract on Ocular Functions of Pups from Diabetic and Hypercholesterolemic Mother Rats PubMed: 21697998
Clinical & Experimental Ophthalmology Analysis of fine structure and biochemical changes of retina during aging of Wistar albino rats Onlinelibrary: ceo.12123
Age (Dordr). Aging-related changes of optic nerve of Wistar albino rats Pubmed:Pmc4039266
Histochem Cell Biol. Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis Pubmed:25511416
Histochem Cell Biol Inhibition of small HA fragment activity and stimulation of A2A PubMed: 25511416
Research Opinions In Animal & Veterinary Sciences Effects of elevated body temperature on structure and function of cerebrum and cerebellum of Wistar male albino rats Pdf-Files: Issue-4-2015
Journal of Molecular Biomarkers J & Diagnosis Role of Pomegranate Juice and Atorvastatin in Ameliorating Spinal Neurotoxicity of Wistar Rats Maternally Fed on Hypercholesterolemic Diet DOI: 10.4172/2155-9929.1000316
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J Chem Neuroanat Synergistic effects of pomegranate juice and atorvastatin for improving cerebellar structure and function of breast-feeding rats maternally fed on a high cholesterol diet Pubmed: 32339653
Egyptian Journal of Basic and Applied Sciences Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar rats
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