ELISA Kit for Vitamin D Receptor (VDR)

NR1I1; Nuclear Receptor Subfamily 1 Group I Member 1; Calcitriol Receptor; 1,25-dihydroxyvitamin D3 receptor

Specificity

This assay has high sensitivity and excellent specificity for detection of Vitamin D Receptor (VDR).
No significant cross-reactivity or interference between Vitamin D Receptor (VDR) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
6 The vitamin D receptor: A therapeutic target for the treatment of breast cancer? Cgi: Content
endocrine-related cancer Vitamin D receptor as a target for breast cancer therapy. pubmed:28213567
Journal of Advanced Research Effect of exercise on serum vitamin D and tissue vitamin Dreceptors in experimentally induced type 2 Diabetes Mellitus. pubmed:27504197
European Journal of Applied Physiology Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain Pubmed:29143122
BBA- Molecular Basis of Disease Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic … Pubmed: 30553018
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus
Journal of Steroid Biochemistry and Molecular Biology Status of vitamin D and the associated host factors in pulmonary tuberculosis patients and their household contacts: a cross sectional study Pubmed: 31255688
Scientific Reports Association of Fok1 VDR polymorphism with Vitamin D and its associated molecules in pulmonary tuberculosis patients and their household contacts Pubmed: 31649297
Research square Vitamin D Status in Dupuytren's Disease: Association with Clinical Status and Vitamin D Receptor Expression
Betok Fish (Anabas testudineus) Oil Decreases Inflammatory Cytokine through Increasing Vitamin D Level in Rats-induced Systemic Lupus Erythematosus
iium medical journal malaysia Positive Correlation between Monocyte-to-Lymphocyte Ratio and C-Reactive Protein in Vitamin D Deficient Preterm Infants with Respiratory Distress Syndrome
Research Square Identification of SNP of VDR and VDBP gene and its Dysregulated pathway through VDR-VDBP interaction network analysis in Vitamin D deficient Infertile …
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