ELISA Kit for Myoglobin (MYO)

PVALB; MB

Specificity

This assay has high sensitivity and excellent specificity for detection of Myoglobin (MYO).
No significant cross-reactivity or interference between Myoglobin (MYO) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Myoglobin (MYO) and the recovery rates were calculated by comparing the measured value to the expected amount of Myoglobin (MYO) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-104 101
EDTA plasma(n=5) 79-98 90
heparin plasma(n=5) 87-98 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myoglobin (MYO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myoglobin (MYO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myoglobin (MYO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-95% 96-103% 78-101% 83-99%
EDTA plasma(n=5) 92-105% 80-105% 96-103% 94-103%
heparin plasma(n=5) 90-101% 80-102% 98-105% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
European Journal of Applied Physiology Acute leukocyte, cytokine and adipocytokine responses to maximal and hypertrophic resistance exercise bouts Pubmed:25145982
International Journal of Molecular Sciences Transcriptional Alterations by Ischaemic Postconditioning in a Pig Infarction Model: Impact on Microvascular Protection Pubmed: 30650650
BIOSENS BIOELECTRON Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA … Pubmed: 30954926
Mikrochim Acta DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label Pubmed: 31123904
ACS Appl Mater Interfaces Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions 34914377
Talanta A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein Pubmed:35714415
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA480Mu02 Recombinant Myoglobin (MYO) Positive Control; Immunogen; SDS-PAGE; WB.
RPA480Mu01 Recombinant Myoglobin (MYO) Positive Control; Immunogen; SDS-PAGE; WB.
PAA480Mu02 Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
PAA480Mu01 Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IP.
LAA480Mu81 FITC-Linked Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC; IF.
LAA480Mu71 Biotin-Linked Polyclonal Antibody to Myoglobin (MYO) WB; IHC; ICC.
SEA480Mu ELISA Kit for Myoglobin (MYO) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA480Mu CLIA Kit for Myoglobin (MYO) Chemiluminescent immunoassay for Antigen Detection.
LMA480Mu Multiplex Assay Kit for Myoglobin (MYO) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.