ELISA Kit for Fibrinogen Like Protein 2 (FGL2)

T49; PT49; Fibroleukin

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 2 (FGL2).
No significant cross-reactivity or interference between Fibrinogen Like Protein 2 (FGL2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibrinogen Like Protein 2 (FGL2) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen Like Protein 2 (FGL2) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 87-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Like Protein 2 (FGL2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Like Protein 2 (FGL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen Like Protein 2 (FGL2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 82-102% 87-102% 78-90% 90-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Gut. Expression of B and T lymphocyte attenuator (BTLA) in macrophages contributes to the fulminant hepatitis caused by murine hepatitis virus strain-3 PubMed: 22637698
Hepatogastroenterology. Plasma fibrinogen-like protein 2 levels in patients with non-alcoholic fatty liver disease. PubMed: 22024080
PLoS Pathogens Programmed Death (PD)-1-Deficient Mice Are Extremely Sensitive to Murine Hepatitis Virus Strain-3 (MHV-3) Infection PubMed: PMC3131267
Immunology letters Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection. Pubmed: 24286726
Immunol Lett. Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection Pubmed:24286726
NATURE COMMUNICATIONS VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism pubmed:29109438
Cancer Medicine Soluble fibrinogen‐like protein 2 ameliorates acute rejection of liver transplantation in rat via inducing Kupffer cells M2 polarization Pubmed:29749104
BMC Infectious Diseases Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases Pubmed: 30419833
Int J Gynaecol Obstet Tandem mass tag©\based quantitative proteomic profiling of the serum of patients with abnormal uterine bleeding associated with copper intrauterine device 33354778
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