ELISA Kit for Cytokeratin 16 (CK16)

KRT16; NEPPK; Keratin 16; Focal Non-Epidermolytic Palmoplantar Keratoderma; Keratin, type I cytoskeletal 16

Specificity

This assay has high sensitivity and excellent specificity for detection of Cytokeratin 16 (CK16).
No significant cross-reactivity or interference between Cytokeratin 16 (CK16) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cytokeratin 16 (CK16) and the recovery rates were calculated by comparing the measured value to the expected amount of Cytokeratin 16 (CK16) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-104 89
EDTA plasma(n=5) 95-105 101
heparin plasma(n=5) 97-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 16 (CK16) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 16 (CK16) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytokeratin 16 (CK16) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-90% 78-99% 84-94% 88-97%
EDTA plasma(n=5) 79-89% 87-96% 83-98% 80-105%
heparin plasma(n=5) 95-102% 89-98% 84-103% 87-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Annals of Anatomy-Anatomischer Anzeiger Normal microscopic anatomy of equine body and limb skin: A morphological and immunohistochemical study Pubmed:29730469
SLAS Discov High-Throughput Screening to Identify Small Molecules That Selectively Inhibit APOL1 Protein Level in Podocytes 34218698
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA516Hu01 Recombinant Cytokeratin 16 (CK16) Positive Control; Immunogen; SDS-PAGE; WB.
PAA516Hu01 Polyclonal Antibody to Cytokeratin 16 (CK16) WB; IHC; ICC; IP.
LAA516Hu71 Biotin-Linked Polyclonal Antibody to Cytokeratin 16 (CK16) WB; IHC; ICC.
LAA516Hu81 FITC-Linked Polyclonal Antibody to Cytokeratin 16 (CK16) WB; IHC; ICC; IF.
MAA516Hu22 Monoclonal Antibody to Cytokeratin 16 (CK16) WB; IHC; ICC; IP.
SEA516Hu ELISA Kit for Cytokeratin 16 (CK16) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA516Hu Multiplex Assay Kit for Cytokeratin 16 (CK16) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.