ELISA Kit for Tissue Factor (TF)

CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III

Specificity

This assay has high sensitivity and excellent specificity for detection of Tissue Factor (TF).
No significant cross-reactivity or interference between Tissue Factor (TF) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Tissue Factor (TF) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Factor (TF) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Factor (TF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 80-104% 80-94% 93-103% 91-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Peptides Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats PubMed: 18625276
Canadian Journal of Physiology and Pharmacology Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor Nrcresearchpress: y10-115
International immunopharmacology Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats Pubmed: 23538027
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239
Hippokratia Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor PubMed: 20011089
shock Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock PubMed: 25423126
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Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502
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Thrombosis andHaemostasis Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome Pubmed: 29202212
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Mediators Inflamm Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB … Pubmed: 32587471
International Journal of Clinical Practice Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation System Pubmed:35685509
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA524Hu01 Recombinant Tissue Factor (TF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA524Hu01 Polyclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IP.
FAA524Hu01 Anti-Tissue Factor (TF) Polyclonal Antibody Flow cytometry.
LAA524Hu71 Biotin-Linked Polyclonal Antibody to Tissue Factor (TF) WB; IHC; ICC.
LAA524Hu81 FITC-Linked Polyclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IF.
FAA524Hu81 FITC-Linked Anti-Tissue Factor (TF) Polyclonal Antibody FCM
MAA524Hu21 Monoclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IP.
MAA524Hu22 Monoclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IP.
MAA524Hu23 Monoclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IP.
FAA524Hu42 PE-Linked Anti-Tissue Factor (TF) Monoclonal Antibody FCM
SEA524Hu ELISA Kit for Tissue Factor (TF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA524Hu CLIA Kit for Tissue Factor (TF) Chemiluminescent immunoassay for Antigen Detection.
LMA524Hu Multiplex Assay Kit for Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.