ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)
EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor
- Product No.SEA552Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Tissue Inhibitors Of Metalloproteinase 1 (TIMP1).
No significant cross-reactivity or interference between Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Experimental & Translational Stroke Medicine||Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke PubMed: 21679435|
|PLoS ONE||A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source|
|Polski Przeglad Chirurgiczny||Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course Pubmed: 23585206|
|Thromb Haemost.||Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655|
|The Scientific World Journal||Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats Pubmed:24701154|
|PLOS ONE||The Complementary Effects of Atorvastatin and Exercise Treatment on the Composition and Stability of the Atherosclerotic Plaques in ApoE Knockout Mice Pubmed:25264981|
|Mediators of Inflammation||Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse Journals: Mi|
|PLOS||Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat PubMed: 25945502|
|Canadian Journal of Physiology and Pharmacology||Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells Doi: Abs|
|PLoS One||Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion PubMed: 26680220|
|Kyselov||Влияние диуретиков с антиальдостероновым действием на состояние межклеточного матрикса и раннее постинфарктное ремоделирование левого … Article: View|
|Оригинальные исследования / Original researches||матриксу та ранн Article: Viewfile|
|Basic Research||CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Journal:27699|
|J Orthop Res.||Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. Pubmed:26748661|
|Int Wound J.||From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748|
|Am J Pathol.||Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Pubmed:26963343|
|Journal of Orthopaedic Research||Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation pubmed:26748661|
|Canadian Journal of Veterinary Research||Evaluation of hyaluronic acid, procollagen type III N-terminal peptide, and tissue inhibitor of matrix metalloproteinase-1 as serum markers of canine hepatic fibrosis pubmed:27733785|
|BMC Veterinary Research||Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. pubmed:27938355|
|Journal of Cellular Physiology||Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models pubmed:27739057|
|Toxicology and Applied Pharmacology||Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) pubmed:28974454|
|Journal of Applied Animal Research||Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis 10.108:09712119.2017.1335208|
|Cellular Physiology and Biochemistry||The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells pubmed:29224019|
|International Wound Journal||From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748|
|Archives of Medical Science||CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. pubmed:28507585|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
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|LAA552Hu71||Biotin-Linked Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)||WB; IHC; ICC.|
|MAA552Hu22||Monoclonal Antibody to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)||WB; IHC; ICC; IP.|
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|SEA552Hu||ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
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