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ELISA Kit for Epidermal Growth Factor (EGF)

URG; Beta-Urogastrone; Pro-epidermal growth factor

Specificity

This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF).
No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Epidermal Growth Factor (EGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Epidermal Growth Factor (EGF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-97 93
EDTA plasma(n=5) 85-98 92
heparin plasma(n=5) 78-90 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Epidermal Growth Factor (EGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 85-97% 98-105% 97-105%
EDTA plasma(n=5) 78-94% 96-104% 98-105% 78-92%
heparin plasma(n=5) 86-96% 85-101% 92-101% 88-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Journal of Pediatric Surgery The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Plos one The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor PubMed: PMC3590171
Diabetes. Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity Pubmed:25795216
Eur Rev Med Pharmacol Sci.? Association of colon adenomas and skin tags: coincidence or coexistence? Pubmed:24763889
Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972
ACS Applied Materials & Interfaces Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. pubmed:27709887
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats pubmed:28177680
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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