ELISA Kit for Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- Product No.SEA560Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range7.8-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.9pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF).
No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Epidermal Growth Factor (EGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Epidermal Growth Factor (EGF) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Epidermal Growth Factor (EGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Pediatric Surgery||The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940|
|Biomark Insights||Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607|
|Plos one||The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor PubMed: PMC3590171|
|Diabetes.||Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity Pubmed:25795216|
|Eur Rev Med Pharmacol Sci.?||Association of colon adenomas and skin tags: coincidence or coexistence? Pubmed:24763889|
|Int J Clin Exp Med.||The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972|
|ACS Applied Materials & Interfaces||Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. pubmed:27709887|
|Canadian Journal of Physiology and Pharmacology||The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats pubmed:28177680|
|Journal of Controlled Release||A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury pubmed:28522195|
|Scientific Reports||Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA560Mu02||Recombinant Epidermal Growth Factor (EGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA560Mu01||Active Epidermal Growth Factor (EGF)||Cell culture; Activity Assays.|
|RPA560Mu01||Recombinant Epidermal Growth Factor (EGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA560Mu02||Polyclonal Antibody to Epidermal Growth Factor (EGF)||WB; IHC; ICC; IP.|
|PAA560Mu01||Polyclonal Antibody to Epidermal Growth Factor (EGF)||WB; IHC; ICC; IP.|
|SEA560Mu||ELISA Kit for Epidermal Growth Factor (EGF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEA560Mu||Mini Samples ELISA Kit for Epidermal Growth Factor (EGF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA560Mu||CLIA Kit for Epidermal Growth Factor (EGF)||Chemiluminescent immunoassay for Antigen Detection.|