ELISA Kit for S100 Calcium Binding Protein A11 (S100A11)

S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11, N-terminally processed

Specificity

This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A11 (S100A11).
No significant cross-reactivity or interference between S100 Calcium Binding Protein A11 (S100A11) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant S100 Calcium Binding Protein A11 (S100A11) and the recovery rates were calculated by comparing the measured value to the expected amount of S100 Calcium Binding Protein A11 (S100A11) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-92 81
EDTA plasma(n=5) 87-94 90
heparin plasma(n=5) 80-101 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A11 (S100A11) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A11 (S100A11) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100 Calcium Binding Protein A11 (S100A11) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 91-99% 90-103% 85-94%
EDTA plasma(n=5) 80-91% 96-105% 99-105% 88-97%
heparin plasma(n=5) 80-102% 83-99% 80-94% 97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis 10.1016:S0016-5085(18)33544-3
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis 10.1016:S0016-5085(18)33544-5
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study Pubmed: 31856598
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA568Mu02 Recombinant S100 Calcium Binding Protein A11 (S100A11) Positive Control; Immunogen; SDS-PAGE; WB.
RPA568Mu01 Recombinant S100 Calcium Binding Protein A11 (S100A11) Positive Control; Immunogen; SDS-PAGE; WB.
PAA568Mu02 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11) WB; IHC; ICC; IP.
PAA568Mu01 Polyclonal Antibody to S100 Calcium Binding Protein A11 (S100A11) WB; IHC; ICC; IP.
SEA568Mu ELISA Kit for S100 Calcium Binding Protein A11 (S100A11) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA568Mu Multiplex Assay Kit for S100 Calcium Binding Protein A11 (S100A11) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.