ELISA Kit for Aquaporin 4 (AQP4)
HMIWC2; MIWC; WCH4; Mercurial-insensitive water channel
- Product No.SEA582Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.112ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Aquaporin 4 (AQP4).
No significant cross-reactivity or interference between Aquaporin 4 (AQP4) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 4 (AQP4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 4 (AQP4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Neuroscience Letters||Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patients ScienceDirect: S0304394011012341|
|Neurology||Astrocytic damage is far more severe than demyelination in NMO Neurology: 208|
|Fluids and Barriers of the CNS||Aquaporin-4 expression in the cerebrospinal fluid in congenital human hydrocephalus PubMed: PMC3651869|
|Neuropeptides||Melanocortin MC4 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat PubMed: 25616531|
|Human & Experimental Toxicology||Novel effect of Daflon and low-dose γ-radiation in modulation of thioacetamide-induced hepatic encephalopathy in male albino rats Pubmed:26987350|
|Cephalalgia||Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertension Pubmed:26853804|
|PLoS One||Cerebrospinal fluid biomarkers of infantile congenital hydrocephalus. pubmed:28212403|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA582Ra01||Recombinant Aquaporin 4 (AQP4)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA582Ra01||Polyclonal Antibody to Aquaporin 4 (AQP4)||WB; IHC; ICC; IP.|
|MAA582Ra21||Monoclonal Antibody to Aquaporin 4 (AQP4)||WB; IHC; ICC; IP.|
|LAA582Ra72||Biotin-Linked Monoclonal Antibody to Aquaporin 4 (AQP4)||WB; IHC; ICC.|
|SEA582Ra||ELISA Kit for Aquaporin 4 (AQP4)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSA582Ra01||Antibody Pair for Aquaporin 4 (AQP4)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSA582Ra01||ELISA Kit DIY Materials for Aquaporin 4 (AQP4)||Main materials for "Do It (ELISA Kit) Yourself".|