ELISA Kit for Heme Oxygenase 1, Decycling (HO1)

HMOX1; Hsp32; HMOX1D


This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 1, Decycling (HO1).
No significant cross-reactivity or interference between Heme Oxygenase 1, Decycling (HO1) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Heme Oxygenase 1, Decycling (HO1) and the recovery rates were calculated by comparing the measured value to the expected amount of Heme Oxygenase 1, Decycling (HO1) in samples.

MatrixRecovery range (%)Average(%)
EDTA plasma(n=5)87-10197
heparin plasma(n=5)85-10189


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 1, Decycling (HO1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 1, Decycling (HO1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heme Oxygenase 1, Decycling (HO1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

EDTA plasma(n=5)78-98%89-99%93-101%98-105%
heparin plasma(n=5)81-94%91-103%94-101%90-98%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



BioMed Research InternationalSerum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing SpondylitisPubmed:27314037
Cancer MedicineCirculating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host diseasePubmed:27109254
Redox Report: Communications in Free Radical ResearchDecreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidityPubmed:26824276
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