ELISA Kit for Heme Oxygenase 1 (HO1)
HMOX1; Hsp32; HMOX1D
- Product No.SEA584Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.065ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 1 (HO1).
No significant cross-reactivity or interference between Heme Oxygenase 1 (HO1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Heme Oxygenase 1 (HO1) and the recovery rates were calculated by comparing the measured value to the expected amount of Heme Oxygenase 1 (HO1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 1 (HO1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 1 (HO1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heme Oxygenase 1 (HO1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|The American Journal of Pathology||Characterization of the Intrarenal Renin-Angiotensin System in Experimental Alport Syndrome PubMed: 25777063|
|BioMed Research International||Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis Pubmed:27314037|
|Cancer Medicine||Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease Pubmed:27109254|
|Redox Report: Communications in Free Radical Research||Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidity Pubmed:26824276|
|Canadian Journal of Physiology and Pharmacology||Sulforaphane protects against sodium valproate–induced acute liver injury pubmed:28112972|
|Peptides.||Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. pubmed:27777064|
|european journal of pharmacology||S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. pubmed:27887948|
|brain research||Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. pubmed:28238669|
|biomedicine & pharmacotherapy||Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. pubmed:28068636|
|Inflammopharmacology.||Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis. pubmed:28050757|
|Environmental Toxicology and Pharmacology||Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study pubmed:28192751|
|Journal of Microbiology, Immunology and Infection||Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573|
|Nutrition & Metabolism||Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolism pubmed:28694840|
|Biomedicine & Pharmacotherapy||Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinoma pubmed:29101811|
|Acta Paediatrica||Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106|
|Naunyn-Schmiedeberg's Arch Pharmacol||Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1 pubmed:28589248|
|Asian Pacific Journal of Cancer Prevention||Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathway pubmed:28670881|
|Biomedicine & pharmacotherapy||Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms pubmed:28061403|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA584Ra01||Recombinant Heme Oxygenase 1 (HO1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA584Ra01||Polyclonal Antibody to Heme Oxygenase 1 (HO1)||WB; IHC; ICC; IP.|
|LAA584Ra71||Biotin-Linked Polyclonal Antibody to Heme Oxygenase 1 (HO1)||WB; IHC; ICC.|
|LAA584Ra81||FITC-Linked Polyclonal Antibody to Heme Oxygenase 1 (HO1)||WB; IHC; ICC; IF.|
|MAA584Ra21||Monoclonal Antibody to Heme Oxygenase 1 (HO1)||WB; IHC; ICC; IP.|
|SEA584Ra||ELISA Kit for Heme Oxygenase 1 (HO1)||Enzyme-linked immunosorbent assay for Antigen Detection.|