ELISA Kit for Caspase 3 (CASP3)

CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3).
No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
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Reprod Sci. Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells. Pubmed: 23420823
European journal of pharmacology The combined effect of metformin and L-cysteine on inflammation, oxidative stress and insulin resistance in streptozotocin-induced type 2 diabetes in rats Pubmed: 23845213
Journal of Physiology and Biochemistry Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats Springer: Source
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829
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Journal of Traditional and Complementary Medicine Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats Sciencedirect:S2225411015000231
J Physiol Biochem. Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats Pubmed:24752458
Molecular Biology Reports Apoptotic effects of dipyrido [3,2-a:2&#039;,3&#039;-c] phenazine (dppz) Au(III) complex against diethylnitrosamine/phenobarbital induced experimental hepatocarcinogenesis in rats Pubmed:24756331
Life Sciences Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats Pubmed:25010842
European Journal of Pharmacology Neuroprotective effects of rosuvastatin against traumatic spinal cord injury in rats Pubmed:25084223
J Investig Clin Dent. Expression of key executioner of apoptosis caspase?\3 in periodontal health and disease Pubmed:25388853
Neuropharmacology Saxagliptin: A novel antiparkinsonian approach Pubmed:25446674
Molecular Neurobiology Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations Pubmed:25502462
Histochem Cell Biol. Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis Pubmed:25511416
Protective role of ellagitannins from Eucalyptus citriodora against ethanol-induced gastric ulcer in rats: Impact on oxidative stress, inflammation and calcitonin-gene related peptide Pubmed:25636864
Biochemical Pharmacology Epigallocatechin-3-gallate pretreatment attenuates doxorubicin-induced cardiotoxicity in rats: A mechanistic study Pubmed:25701654
Journal of Pharmacology Research CILOSTAZOL HEPATOPROTECTIVE EFFECT AGAINST ISCHEMIA/REPERFUSION: INVOLVEMENT OF GSK-3β, CYCLIN D1 and WNT/β-CATENIN PATHWAY Researchgate:Source
Life Sciences Attenuation of renal ischemia/reperfusion injury by a PubMed: 25476829
Injury Neuroprotective PubMed: 26298021
PLOS ONE Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug PubMed: 26262991
Histochem Cell Biol Inhibition of small HA fragment activity and stimulation of A2A PubMed: 25511416
PLOS ONE BDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 Cells PubMed: 25749582
Life Sci Geraniol ameliorates TNBS-induced colitis: Involvement of Wnt/棺-catenin, p38MAPK, NF觀B, and PPAR款 signaling pathways PubMed: 26165751
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Pharmacological Reports The heat shock protein 90 inhibitor, 17-AAG, attenuates thioacetamide induced liver fibrosis in mice Science: Article
European Journal of Medicinal Plants Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanisms openview:626440
Chemico-Biological Interactions Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways Pubmed:26807765
Oncotarget An integrated proteomics approach shows synaptic plasticity changes in an APP/PS1 Alzheimers mouse model Pubmed:27144524
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Journal of Natural Medicines Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice Pubmed:26972100
Iranian Journal of Basic Medical Sciences Neuroprotective effects of atomoxetine against traumatic spinal cord injury in rats pmc:PMC4834117
IJBPAS THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS publication:298838934
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Original Investigation Neuroprotective Effects of Pregabalin Against Spinal Cord Ischemia-Reperfusion Injury in Rats JTNEPUB:17959
Molecules Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway Pubmed:27248986
Springerplus. The hepatocurative effects of Cynara scolymus L. leaf extract on carbon tetrachloride-induced oxidative stress and hepatic injury in rats Pubmed:27026910
Oncotarget Chronic low-dose-rate ionising radiation affects the hippocampal phosphoproteome in the ApoE−/− alzheimer mouse model pubmed:27708245
Journal of traditional and complementary medicine. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. pubmed:27774414
pharmaceutical biology Protective effects of apigenin and myricetin against cisplatin-induced nephrotoxicity in mice. pubmed:28064632
Toxicology and Applied Pharmacology Dapoxetine attenuates testosterone-induced prostatic hyperplasia in rats by the regulation of inflammatory and apoptotic proteins pubmed:27687055
Journal of Natural Medicines Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice. pubmed:26972100
BMC Cancer Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. pubmed:27461108
Neurochemical Research Arecoline Induces Neurotoxicity to PC12 Cells: Involvement in ER Stress and Disturbance of Endogenous H2S Generation. pubmed:27255601
Molecular Medicine Reports Hydrogen sulfide attenuates doxorubicin-induced cardiotoxicity by inhibiting the expression of peroxiredoxin III in H9c2 cells mmr:13
The American Journal of Chinese Medicine Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells pubmed:28231747
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. pubmed:28187731
Journal of Ethnopharmacology Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction bydanhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms. pubmed:27729285
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