ELISA Kit for Midkine (MK)

MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2

Specificity

This assay has high sensitivity and excellent specificity for detection of Midkine (MK).
No significant cross-reactivity or interference between Midkine (MK) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Midkine (MK) and the recovery rates were calculated by comparing the measured value to the expected amount of Midkine (MK) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-99 91
EDTA plasma(n=5) 86-99 96
heparin plasma(n=5) 87-103 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Midkine (MK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Midkine (MK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Midkine (MK) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 89-97% 82-93% 80-97%
EDTA plasma(n=5) 78-93% 97-104% 81-103% 90-98%
heparin plasma(n=5) 87-99% 89-98% 90-98% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Midkine: From Embryogenesis to Pathogenesis and Therapy Immunochemical Methods for the Detection of Midkine in Samples of Human Origin Springer: Source
33 Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … 10.1016:j.ebiom.2017.11.020
Circulation Journal Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice pubmed:28781288
EBioMedicine Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … Pubmed:29233575
Journal of Clinical Laboratory Analysis Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall … Pubmed:29797475
Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese :
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA631Mu01 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
RPA631Mu02 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
PAA631Mu01 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
PAA631Mu02 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
LAA631Mu71 Biotin-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC.
LAA631Mu81 FITC-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IF.
SEA631Mu ELISA Kit for Midkine (MK) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA631Mu Multiplex Assay Kit for Midkine (MK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA631Mu01 ELISA Kit DIY Materials for Midkine (MK) Main materials for "Do It (ELISA Kit) Yourself".