ELISA Kit for Receptor For Advanced Glycation Endproducts (RAGE)

AGER; Receptor For Advanced Glycation Endproducts

Specificity

This assay has high sensitivity and excellent specificity for detection of Receptor For Advanced Glycation Endproducts (RAGE).
No significant cross-reactivity or interference between Receptor For Advanced Glycation Endproducts (RAGE) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Receptor For Advanced Glycation Endproducts (RAGE) and the recovery rates were calculated by comparing the measured value to the expected amount of Receptor For Advanced Glycation Endproducts (RAGE) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-90 85
EDTA plasma(n=5) 79-103 88
heparin plasma(n=5) 81-89 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor For Advanced Glycation Endproducts (RAGE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor For Advanced Glycation Endproducts (RAGE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Receptor For Advanced Glycation Endproducts (RAGE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-104% 90-102% 86-95% 98-105%
EDTA plasma(n=5) 99-105% 79-105% 85-102% 81-91%
heparin plasma(n=5) 95-103% 88-103% 86-95% 87-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Taiwanese Journal of Obstetrics & Gynecology HMGB1-RAGE signaling pathway in pPROM Pubmed:29673663
Journal of Medical Imaging and Health Informatics, Chest X-ray Image Analysis and Penehyclidine Hydrochloride Postconditioning Influence of Lung Ischemia Reperfusion Injury
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA645Mu02 Recombinant Receptor For Advanced Glycation Endproducts (RAGE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA645Mu01 Recombinant Receptor For Advanced Glycation Endproducts (RAGE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA645Mu01 Polyclonal Antibody to Receptor For Advanced Glycation Endproducts (RAGE) WB; IHC; ICC; IP.
PAA645Mu02 Polyclonal Antibody to Receptor For Advanced Glycation Endproducts (RAGE) WB; IHC; ICC; IP.
SEA645Mu ELISA Kit for Receptor For Advanced Glycation Endproducts (RAGE) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA645Mu CLIA Kit for Receptor For Advanced Glycation Endproducts (RAGE) Chemiluminescent immunoassay for Antigen Detection.
LMA645Mu Multiplex Assay Kit for Receptor For Advanced Glycation Endproducts (RAGE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.