ELISA Kit for Procalcitonin (PCT)

Pro-Calcitonin

Specificity

This assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT).
No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Procalcitonin (PCT) and the recovery rates were calculated by comparing the measured value to the expected amount of Procalcitonin (PCT) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)81-9994
EDTA plasma(n=5)94-10298
heparin plasma(n=5)79-9383

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procalcitonin (PCT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procalcitonin (PCT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procalcitonin (PCT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)99-105%91-102%80-101%87-101%
EDTA plasma(n=5)82-89%95-102%80-104%92-101%
heparin plasma(n=5)90-104%97-104%80-94%83-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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MagazineReference
Scandinavian Journal of Infectious DiseasesEvaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsisPubMed: 20085423
International Journal of Radiation Oncology ? Biology ? Physics Effects of Berberine Against Radiation-Induced Intestinal Injury in MicePubMed: 20637981
Ulus Travma Acil Cerrahi DergDiagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemiaPubMed: 21935794
Egyptian Journal of BronchologySerum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain childrenEssbronchology: Source
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in BronchiectasisWaikato:Source
Egyptian Journal of Medical MicrobiologyEvaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal SepsisEjmm:Source
Nigerian Medical JournalEfficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsisPubmed:25657488
Wiley online libraryThe use and value of procalcitonin in solid organ transplantationPubMed: 25996831
The Journal of Clinical Endocrinology & MetabolismMacrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassayPubMed: 26647152
Journal of Human HypertensionProcalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertensionPubMed: 26040439
Acta MedicaRisk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy.pubmed:27638962
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