ELISA Kit for Procalcitonin (PCT)
- Product No.SEA689Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT).
No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Procalcitonin (PCT) and the recovery rates were calculated by comparing the measured value to the expected amount of Procalcitonin (PCT) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procalcitonin (PCT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procalcitonin (PCT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procalcitonin (PCT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Scandinavian Journal of Infectious Diseases||Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis PubMed: 20085423|
|International Journal of Radiation Oncology ? Biology ? Physics||Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice PubMed: 20637981|
|Ulus Travma Acil Cerrahi Derg||Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia PubMed: 21935794|
|Egyptian Journal of Bronchology||Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children Essbronchology: Source|
|University of Waikato||The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis Waikato:Source|
|Egyptian Journal of Medical Microbiology||Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis Ejmm:Source|
|Nigerian Medical Journal||Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis Pubmed:25657488|
|Wiley online library||The use and value of procalcitonin in solid organ transplantation PubMed: 25996831|
|Balkan Med J||Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia PubMed: 26185718|
|The Journal of Clinical Endocrinology & Metabolism||Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay PubMed: 26647152|
|Journal of Human Hypertension||Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension PubMed: 26040439|
|Medical Science Monitor||Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection. Pubmed:26772168|
|International Immunopharmacology||Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602|
|Pancreatology.||Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. Pubmed:26804005|
|Acta Medica||Risk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy. pubmed:27638962|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA689Ra01||Recombinant Procalcitonin (PCT)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA689Ra01||Polyclonal Antibody to Procalcitonin (PCT)||WB; IHC; ICC; IP.|
|MAA689Ra21||Monoclonal Antibody to Procalcitonin (PCT)||WB; IHC; ICC; IP.|
|SEA689Ra||ELISA Kit for Procalcitonin (PCT)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA689Ra01||ELISA Kit DIY Materials for Procalcitonin (PCT)||Main materials for "Do It (ELISA Kit) Yourself".|
|PGA689Ra01||Primer Pair for Procalcitonin (PCT)||PCR|