ELISA Kit for Lactoferrin (LTF)
HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
- Product No.SEA780Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.135ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Lactoferrin (LTF).
No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Lactoferrin (LTF) and the recovery rates were calculated by comparing the measured value to the expected amount of Lactoferrin (LTF) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lactoferrin (LTF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|The International Journal of Biochemistry & Cell Biology||Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction ScienceDirect: S1357272511002524|
|BMC Veterinary Research||Changes in composition of colostrum of Egyptian buffaloes and Holstein cows BioMed: 17466148|
|Plos One||p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source|
|The Journal of Biological Chemistry||Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239|
|PLoS One||Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 Pubmed:26974438|
|Bioscience, Biotechnology, and Biochemistry||Salivary lactoferrin is transferred into the brain via the sublingual route pubmed:28351211|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|NPA780Hu01||Native Lactoferrin (LTF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA780Hu01||Active Lactoferrin (LTF)||Cell culture; Activity Assays.|
|RPA780Hu01||Recombinant Lactoferrin (LTF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA780Hu02||Recombinant Lactoferrin (LTF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA780Hu01||Polyclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC; IP.|
|PAA780Hu02||Polyclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC; IP.|
|PAA780Hu05||Polyclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC; IP.|
|LAA780Hu81||FITC-Linked Polyclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC; IF.|
|LAA780Hu71||Biotin-Linked Polyclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC.|
|MAA780Hu22||Monoclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC; IP.|
|LAA780Hu72||Biotin-Linked Monoclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC.|
|LAA780Hu82||FITC-Linked Monoclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC; IF.|
|SEA780Hu||ELISA Kit for Lactoferrin (LTF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA780Hu||CLIA Kit for Lactoferrin (LTF)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA780Hu01||ELISA Kit DIY Materials for Lactoferrin (LTF)||Main materials for "Do It (ELISA Kit) Yourself".|