ELISA Kit for C Reactive Protein (CRP)
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
- Product No.SEA821Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range62.5-4,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 27.7pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
Ensure in stock; if not, free redo!
For negotiated price and more details, please contact local distributors! US$ 455
For negotiated price and more details, please contact local distributors! US$ 2048
For negotiated price and more details, please contact local distributors! US$ 3868
For negotiated price and more details, please contact local distributors! US$ 31850
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP).
No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant C Reactive Protein (CRP) and the recovery rates were calculated by comparing the measured value to the expected amount of C Reactive Protein (CRP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C Reactive Protein (CRP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journalof Vascular Research||Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators Karger: 000257340|
|Journal of Pharmacology and Experimental Therapeutics||Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar Macrophages Aspet: 171462|
|Experimental Animals||Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction Jstage: source|
|Neuroscience||In vivo protective effect of ferulic acid against noise-induced hearing loss in the guinea-pig PubMed: 20600667|
|Heart and Vessels||Metformin inhibits nuclear factor κB activation and decreases serum high-sensitivity C-reactive protein level in experimental atherogenesis of rabbits SpringerLink: pt732478620w1528|
|Plos one||Olive (Olea europaea L.) Leaf Polyphenols Improve Insulin Sensitivity in Middle-Aged Overweight Men: A Randomized, Placebo-Controlled, Crossover Trial PubMed: 23516412|
|Psychosomatic Medicine||Unpredictable chronic mild stress promotes atherosclerosis in high cholesterol-fed rabbits PubMed: 22753638|
|PLOS ONE||Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice PlosOne: Source|
|BMC Neurology||Relationships among serum C-reactive protein, receptor for advanced glycation products, metabolic dysfunction, and cognitive impairments PubMed: PMC3765839|
|Particle and fibre toxicology,||Impact of experimental type 1 diabetes mellitus on systemic and coagulation vulnerability in mice acutely exposed to diesel exhaust particles PubMed: PMC3641025|
|Clinics||Changes in body weight, C-reactive protein, and total adiponectin in non-obese women after 12 months of a small-volume, home-based exercise program PubMed: PMC3752622|
|PLoS One||Pre-Pubertal Children Born Post-Term Have Reduced Insulin Sensitivity and Other Markers of the Metabolic Syndrome PubMed: PMC3698136|
|Scientific Report||Among overweight middle-aged men, first-borns have lower insulin sensitivity than second-borns Pubmed: 24503677|
|International Urology Nephrology||Perirenal fat associated with microalbuminuria in obese rats Pubmed: 24526332|
|AJBM||Effect of caffeic acid on doxorubicin induced cardiotoxicity in rats Ajbm: Source|
|Journal of Molecular and Cellular Cardiology||Effect of high free fatty acids on the anti-contractile response of perivascular adipose tissue in rat aorta Pubmed: 23939490|
|PLoS One.||Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal Microbiota Plos: Source|
|Fundamental & Clinical Pharmacology||The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamol Onlinelibrary: fcp.12074|
|Mainz||Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen Info:Source|
|Int J Clin Exp Med.||Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model Pubmed:Pmc4132129|
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|Nutr Res.||Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits Pubmed:25282128|
|Journal of Obstetrics and Gynaecology Research||Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartan Pubmed:25302540|
|Chemical Research in Toxicology||Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880|
|Journal of Applied Toxicology||Bisphenol A exposure induces metabolic disorders and enhances atherosclerosis in hyperlipidemic rabbits Pubmed:25619500|
|Cellular Physiology and Biochemistry||Short-Term Nose-Only Water-Pipe (Shisha) Smoking Exposure Accelerates Coagulation and Causes Cardiac Inflammation and Oxidative Stress in Mice Pubmed:25634761|
|Food and Chemical Toxicology||Consumption of orange fermented beverage reduces cardiovascular risk factors in healthy mice Pubmed:25666657|
|Cellular Physiology and Biochemistry||Induction of Haemeoxygenase-1 Improves FFA-Induced Endothelial Dysfunction in Rat Aorta Pubmed:25766533|
|Mediators of Inflammation||Effects of Intravenous Injection of PubMed: 26063970|
|Am J Physiol Heart Circ Physiol||Irisin improves endothelial function in obese mice through the AMPK-eNOS pathway PubMed: 26371167|
|Cell Physiol Biochem||Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway PubMed: 26160485|
|Exp Physiol||Renal protective effects of induction of haem oxygenase-1 combined with increased adiponectin on the glomerular vascular endothelial growth factor–nitric oxide axis in obese rats PubMed: 25959017|
|Clin Oral Investig||Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? PubMed: 26556576|
|Chemical Research in Toxicology||Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880|
|Acta Biomater||Resveratrol Inhibited Inflammation andAlveolar Bone Loss in Periodontitis PubMed: 26497626|
|Archives of Physiology and Biochemistry||Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364|
|Journal of Functional Foods||Orange beverage ameliorates high-fat-diet-induced metabolic disorder in mice article:S1756464616300688|
|Medical Science Monitor||Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection. Pubmed:26772168|
|J Diabetes Res.||Central Administration of Galanin Receptor 1 Agonist Boosted Insulin Sensitivity in Adipose Cells of Diabetic Rats pmc:PMC4835658|
|Asian J Pharm Clin Res||THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS journals:10835|
|J Bone Miner Res||Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082|
|Naunyn-Schmiedeberg's Archives of Pharmacology||Combined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expression pubmed:27447455|
|Journal of Coastal Life Medicine||Cardioprotective and renoprotective effects of Cocos nucifera water in offspring of high fat diet fed Wistar rat dams qk:20168|
|Journal of Functional Foods||Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolism S1756464617300890|
|Environmental toxicology and pharmacology||Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. pubmed:28376377|
|Andrology||Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function through increased endothelial dysfunction, oxidative stress, apoptosis, and corporal fibrosis … doi:10.1111|
|Journal of Diabetes Research||Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose pubmed:27127795|
|Therapeutics and Clinical Risk Management||Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. pubmed:27540296|
|Veterinary World||Acute phase response in lame crossbred dairy cattle pubmed:27956769|
|J Ethnopharmacol||Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis. pubmed:27880884|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
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|PAA821Hu02||Polyclonal Antibody to C Reactive Protein (CRP)||WB; IHC; ICC; IP.|
|PAA821Hu01||Polyclonal Antibody to C Reactive Protein (CRP)||WB; IHC; ICC; IP.|
|LAA821Hu71||Biotin-Linked Polyclonal Antibody to C Reactive Protein (CRP)||WB; IHC; ICC.|
|MAA821Hu22||Monoclonal Antibody to C Reactive Protein (CRP)||WB; IHC; ICC; IP.|
|LAA821Hu72||Biotin-Linked Monoclonal Antibody to C Reactive Protein (CRP)||WB; IHC; ICC.|
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|SEA821Hu||ELISA Kit for C Reactive Protein (CRP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
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|SCA821Hu||CLIA Kit for C Reactive Protein (CRP)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA821Hu01||ELISA Kit DIY Materials for C Reactive Protein (CRP)||Main materials for "Do It (ELISA Kit) Yourself".|