ELISA Kit for Follicle Stimulating Hormone (FSH)
- Product No.SEA830Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78-50mIU/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.34mIU/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 520
For more details, please contact local distributors! US$ 2340
For more details, please contact local distributors! US$ 4420
For more details, please contact local distributors! US$ 36400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone (FSH).
No significant cross-reactivity or interference between Follicle Stimulating Hormone (FSH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Follicle Stimulating Hormone (FSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Follicle Stimulating Hormone (FSH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follicle Stimulating Hormone (FSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|International Journal of Clinical and Experimental Medicine||Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Ijcem: IJCEM1102004|
|Science Alert||Reproductive Toxicity of Tobacco Shisha Smoking on Semen Parameters and Hormones Levels among Adult Egyptian Men SciAlert: source|
|EXCLI Journal||Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats Excli: Source|
|International Journal of Molecular and Cellular Medicine||Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777|
|Urologia internationalis||Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982|
|Electromagnetic Biology and Medicine||The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source|
|Academic Sciences||Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source|
|Mol Cell Endocrinol.||Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats Pubmed:24316378|
|Endocrinology||FoxO1 Is a Negative Regulator of FSHβ Gene Expression in Basal and GnRH-Stimulated Conditions in Female Pubmed:24437485|
|Andrologia||Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis Pubmed:24576220|
|Environmental Toxicology and Pharmacology||Endocrine disrupting effects of dichlorodiphenyltrichloroethane analogues on gonadotropin hormones in pituitary gonadotrope cells Pubmed:24814263|
|Environmental Research||Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141|
|Genet Mol Biol.||Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846|
|Turkish Journal of Urology||Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source|
|oncology research||Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940|
|Andrologia||Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis PubMed: 24576220|
|European Journal of Pharmacology||The effect of R-(-)-deprenyl administration on reproductive parameters of rat males PubMed: 25725114|
|Andrologia||Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats PubMed: 26123866|
|Reprod Toxicol||Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats PubMed: 26476359|
|The Journal of The Society for Reproduction and Fertility||Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure Pubmed:27026714|
|Biology of Reproduction||Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114|
|Andrologia||Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. Pubmed:26123866|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
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|environmental pollution||Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061|
|Andrologia||Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943|
|Biology of Reproduction||Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. pubmed:26962114|
|J Endocrinol.||Impact of uteroplacental insufficiency on postnatal rat male gonad. pubmed:27885054|
|Int J Pharm.||New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. pubmed:28232269|
|Environmental Science & Technology||Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686|
|Journal of Medicinal Chemistry||Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity pubmed:28958147|
|Society of Toxicology||Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells pubmed:29149324|
|Reproductive Toxicology||Vitamin D deficiency impairs testicular development and spermatogenesis in mice pubmed:28655646|
|The Journal of Biological Chemistry.||Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. pubmed:28578315|
|The Journal of Toxicological Sciences||High sensitivity of testicular function to titanium nanoparticles pubmed:28496042|
|Journal of Orthopaedic Research||The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779|
|DARU Journal of Pharmaceutical Sciences||Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999|
|Reproduction.||Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593|
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