ELISA Kit for Renin (REN)
- Product No.SEA889Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 11.6pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 480
For more details, please contact local distributors! US$ 2160
For more details, please contact local distributors! US$ 4080
For more details, please contact local distributors! US$ 33600
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Renin (REN).
No significant cross-reactivity or interference between Renin (REN) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Renin (REN) and the recovery rates were calculated by comparing the measured value to the expected amount of Renin (REN) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renin (REN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renin (REN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Renin (REN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Hypertension||Prenatal overexposure to glucocorticoids programs renal 11β-hydroxysteroid dehydrogenase type 2 expression and salt-sensitive hypertension in the rat PubMed: 21045727|
|The Journal of Physiology||Recombinant human erythropoietin in humans down-regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate Physoc: 194241|
|The Journal of Physiology||Erythropoietin down‐regulates proximal renal tubular reabsorption and causes a fall in glomerular filtration rate in humans PubMed: 20724370|
|Pathobiology||Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer Cells Karger: 000330169|
|Journal of Cardiovascular Pharmacology||The Angiotensin Receptor Blocker, Azilsartan Medoxomil (TAK-491), Suppresses Vascular Wall Expression of Plasminogen Activator Inhibitor Type-I Protein Potentially Facilitating the Stabilization of Atherosclerotic Plaques LWW: 08000|
|Kidney International||Multiple and large simple renal cysts are associated with prehypertension and hypertension. Pubmed: 23389415|
|Human Physiology||Estimation of the parameters of the renin-angiotensin-aldosterone system, water-electrolyte metabolism, and endothelial function in newborns of women with chronic hypertension Springer:Source|
|Journal of Pharmacological Sciences||Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats Pubmed:24366191|
|Liver International||VSL#3 probiotic treatment decreases bacterial translocation in rats with carbon tetrachloride-induced cirrhosis Pubmed:24750552|
|Gynecological Endocrinology||Immunological and hemostasiological disorders in women with ovarian hyperstimulation syndrome Pubmed:25200828|
|Oxidative Medicine and Cellular Longevity||Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice Pubmed:27200147|
|PLoS One||Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria Pubmed:27166674|
|PLOS ONE||Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. pubmed:27391973|
|Heart Lung Circ.||Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. pubmed:27555054|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA889Hu01||Recombinant Renin (REN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA889Hu01||Polyclonal Antibody to Renin (REN)||WB; IHC; ICC; IP.|
|MAA889Hu22||Monoclonal Antibody to Renin (REN)||WB; IHC; ICC; IP.|
|SEA889Hu||ELISA Kit for Renin (REN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA889Hu||CLIA Kit for Renin (REN)||Chemiluminescent immunoassay for Antigen Detection.|