ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4)
PRBP; RBP; Plasma retinol-binding protein
- Product No.SEA929Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.625-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.292ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Retinol Binding Protein 4, Plasma (RBP4).
No significant cross-reactivity or interference between Retinol Binding Protein 4, Plasma (RBP4) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Retinol Binding Protein 4, Plasma (RBP4) and the recovery rates were calculated by comparing the measured value to the expected amount of Retinol Binding Protein 4, Plasma (RBP4) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 4, Plasma (RBP4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 4, Plasma (RBP4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Retinol Binding Protein 4, Plasma (RBP4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Diabetes Technology & Therapeutics||Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078|
|Journal of Pharmaceutical and Biomedical Analysis||Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593|
|Evid Based Complement Alternat Med||Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats PubMed: PMC3368504|
|Journal of Endocrinology||Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles Pubmed:24501381|
|J Endocrinol||Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061|
|Peptides||Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats Pubmed:25445608|
|Obesity Surgery||Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126|
|Clin Exp Nephrol||Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome PubMed: 26351173|
|Experimental and Therapeutic Medicine||Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone Pubmed:27446311|
|Experimental and Therapeutic Medicine||Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. pubmed:27446311|