ELISA Kit for Intelectin 1 (ITLN1)

HL1; INTL; ITLN; LFR; HIntL; Omentin; Intelectin 1,Galactofuranose Binding; Intestinal Lactoferrin Receptor;Endothelial lectin HL-1

Specificity

This assay has high sensitivity and excellent specificity for detection of Intelectin 1 (ITLN1).
No significant cross-reactivity or interference between Intelectin 1 (ITLN1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Intelectin 1 (ITLN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Intelectin 1 (ITLN1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-91 82
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 85-98 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intelectin 1 (ITLN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intelectin 1 (ITLN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Intelectin 1 (ITLN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 98-105% 98-105% 85-92%
EDTA plasma(n=5) 84-91% 93-101% 83-98% 80-101%
heparin plasma(n=5) 87-94% 80-94% 99-105% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Plos one Cardioprotective Properties of Omentin-1 in Type 2 Diabetes: Evidence from Clinical and In Vitro Studies PubMed: PMC3612072
Rev Port Cardiol. Serum visfatin and omentin levels in slow coronary flow Pubmed:25481776
Thieme Evaluation of the Relation Between Omentin-1 and Vitamin D in Postmenopausal Women With or Without Osteoporosis DOI: 10.1055/s-0043-120110
Archives of Gynecology and Obstetrics Maternal and neonatal omentin-1 levels in gestational diabetes Pubmed:29335783
Journal of Cardiovascular Translational Research Long-Term Weight Gain Associated With High Omentin Levels at Hospital Discharge Improves Prognosis of Patients Following Acute Heart Failure Pubmed: 30353296
Probiotics and Antimicrobial Proteins Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar Pubmed: 30232744
Iranian Journal of Pediatrics The Comparison of Cord Blood Omentin-1 Values of Newborns in the Different Birth Weights According to Gestational Age
Mucosal Immunology ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism Pubmed: 31278375
Nature Communications ITLN1 modulates invasive potential and metabolic reprogramming of ovarian cancer cells in omental microenvironment Pubmed: 32669559
A New Biomarker Tool for Risk Stratification in ¡°De Novo¡± Acute Heart Failure (OROME)
cells The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure Pubmed:35455943
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA933Mu01 Recombinant Intelectin 1 (ITLN1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA933Mu02 Recombinant Intelectin 1 (ITLN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA933Mu02 Polyclonal Antibody to Intelectin 1 (ITLN1) WB; IHC; ICC; IP.
PAA933Mu01 Polyclonal Antibody to Intelectin 1 (ITLN1) WB; IHC; ICC; IP.
LAA933Mu71 Biotin-Linked Polyclonal Antibody to Intelectin 1 (ITLN1) WB; IHC; ICC.
SEA933Mu ELISA Kit for Intelectin 1 (ITLN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA933Mu Multiplex Assay Kit for Intelectin 1 (ITLN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.