ELISA Kit for Trefoil Factor 1 (TFF1)

BCEI; HP1.A; HPS2; PS2; PNR2; Breast Cancer,Estrogen-Inducible Sequence Expressed In; Breast cancer estrogen-inducible protein; Polypeptide P1.A

Specificity

This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 1 (TFF1).
No significant cross-reactivity or interference between Trefoil Factor 1 (TFF1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Trefoil Factor 1 (TFF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Trefoil Factor 1 (TFF1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-101 93
EDTA plasma(n=5) 96-104 99
heparin plasma(n=5) 82-99 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Trefoil Factor 1 (TFF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 78-94% 87-94% 90-98%
EDTA plasma(n=5) 92-99% 98-105% 89-96% 96-103%
heparin plasma(n=5) 86-101% 80-92% 84-94% 88-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
BMC Gastroenterology Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China Biomedcentral: Source
Biochimica et Biophysica Acta miR218-5p regulates the proliferation of gastric cancer cells by targeting TFF1 in an Erk1/2-dependent manner Pubmed:25652124
clinical cancer research Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma PubMed: 26240291
BMC Cancer miR-632 promotes gastric cancer progression by accelerating angiogenesis in a TFF1-dependent manner Pubmed: 30612555
Nutrients Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation Pubmed: 32784583
International Journal of Molecular Sciences Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
PLOS MEDICINE A combination of urinary biomarker panel and PancRISK score for earlier detection of pancreatic cancer: A case¨Ccontrol study 33301466
Int. J. Mol. Sci. Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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