ELISA Kit for Fibrinogen Alpha Chain (FGA)

Fib2; FG-A; FGA; Fibrinopeptide A

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibrinogen Alpha Chain (FGA).
No significant cross-reactivity or interference between Fibrinogen Alpha Chain (FGA) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibrinogen Alpha Chain (FGA) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen Alpha Chain (FGA) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 92-105 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Alpha Chain (FGA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Alpha Chain (FGA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen Alpha Chain (FGA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 92-105% 97-105% 90-101% 79-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
PLoS One Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis PubMed: 25789864
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
GLYCOCONJUGATE JOURNAL Nattokinase-heparin exhibits beneficial efficacy and safety—an optimal strategy for CKD patients on hemodialysis Pubmed: 30788657
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy Pubmed: 31162828
Reproductive BioMedicine Online Fibrinogen alpha chain is upregulated and affects the pathogenesis of endometriosis
Redox Biology Breaking the vicious loop between inflammation, oxidative stress and coagulation, a novel anti-thrombus insight of nattokinase by inhibiting LPS-induced … Pubmed: 32193146
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB154Ra02 Recombinant Fibrinogen Alpha Chain (FGA) Positive Control; Immunogen; SDS-PAGE; WB.
RPB154Ra01 Recombinant Fibrinogen Alpha Chain (FGA) Positive Control; Immunogen; SDS-PAGE; WB.
PAB154Ra01 Polyclonal Antibody to Fibrinogen Alpha Chain (FGA) WB; IHC; ICC; IP.
PAB154Ra02 Polyclonal Antibody to Fibrinogen Alpha Chain (FGA) WB; IHC; ICC; IP.
LAB154Ra71 Biotin-Linked Polyclonal Antibody to Fibrinogen Alpha Chain (FGA) WB; IHC; ICC.
LAB154Ra81 FITC-Linked Polyclonal Antibody to Fibrinogen Alpha Chain (FGA) WB; IHC; ICC; IF.
MAB154Ra21 Monoclonal Antibody to Fibrinogen Alpha Chain (FGA) WB; IHC; ICC; IP.
MAB154Ra22 Monoclonal Antibody to Fibrinogen Alpha Chain (FGA) WB; IHC; ICC; IP.
SEB154Ra ELISA Kit for Fibrinogen Alpha Chain (FGA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB154Ra Multiplex Assay Kit for Fibrinogen Alpha Chain (FGA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB154Ra01 ELISA Kit DIY Materials for Fibrinogen Alpha Chain (FGA) Main materials for "Do It (ELISA Kit) Yourself".