ELISA Kit for Clusterin (CLU)
CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40,Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2
- Product No.SEB180Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range18.8-1,200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 7.1ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 498
For more details, please contact local distributors! US$ 2241
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For more details, please contact local distributors! US$ 34860
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Clusterin (CLU).
No significant cross-reactivity or interference between Clusterin (CLU) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Clusterin (CLU) and the recovery rates were calculated by comparing the measured value to the expected amount of Clusterin (CLU) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clusterin (CLU) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clusterin (CLU) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Clusterin (CLU) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Endocrinology||Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice Pubmed: 24684302|
|J Mol Neurosci.||Time-Dependent Decrease of Clusterin as a Potential Cerebrospinal Fluid Biomarker for Drug-Resistant Epilepsy. Pubmed: 24488574|
|PLOS ONE||Serum Amyloid A and Clusterin as Potential Predictive Biomarkers for Severe Hand, Foot and Mouth Disease by 2D-DIGE Proteomics Analysis Pubmed:25268271|
|journal of immunological methods||Proteomic analysis of human plasma and peripheral blood mononuclear cells in Systemic Lupus Erythematosus patients. pubmed:28390925|
|Journal of Alzheimer's Disease||Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. pubmed:27911324|
|Biochimica Et Biophysica Acta||Regulatory effects of simvastatin and apoJ on APP processing and amyloid-β clearance in blood-brain barrier endothelial cells pubmed:28941799|
|World Neurosurgery||Clusterin, a New Cerebrospinal Fluid Biomarker in Severe Subarachnoid Hemorrhage: A Pilot Study pubmed:28803177|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB180Mu02||Recombinant Clusterin (CLU)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB180Mu01||Recombinant Clusterin (CLU)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB180Mu01||Polyclonal Antibody to Clusterin (CLU)||WB; IHC; ICC; IP.|
|PAB180Mu02||Polyclonal Antibody to Clusterin (CLU)||WB; IHC; ICC; IP.|
|LAB180Mu81||FITC-Linked Polyclonal Antibody to Clusterin (CLU)||WB; IHC; ICC; IF.|
|LAB180Mu71||Biotin-Linked Polyclonal Antibody to Clusterin (CLU)||WB; IHC; ICC.|
|SEB180Mu||ELISA Kit for Clusterin (CLU)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB180Mu01||ELISA Kit DIY Materials for Clusterin (CLU)||Main materials for "Do It (ELISA Kit) Yourself".|