ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)
HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8
- Product No.SEB286Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.8pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Milk Fat Globule EGF Factor 8 (MFGE8).
No significant cross-reactivity or interference between Milk Fat Globule EGF Factor 8 (MFGE8) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Milk Fat Globule EGF Factor 8 (MFGE8) and the recovery rates were calculated by comparing the measured value to the expected amount of Milk Fat Globule EGF Factor 8 (MFGE8) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Milk Fat Globule EGF Factor 8 (MFGE8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Fertility and Sterility||Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells. Pubmed: 24262600|
|U.S. Patent Application||Biomarker for amyloid-b-related neurological disorders US20130302838|
|IUBMB Life.||Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status. pubmed:28067023|
|Journal of diabetes investigation||Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus. pubmed:28035763|
|The Journal of Steroid Biochemistry and Molecular Biology||Modifications of Western-type diet regarding protein, fat and sucrose levels as modulators of steroid metabolism and activity in liver pubmed:27471150|
|Journal of Steroid Biochemistry and Molecular Biology||Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910|
|Oncotarget||Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response. pubmed:28489606|
|Molecular and Cellular Neuroscience||TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway 10.1016/j.mcn.2017.08.006|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB286Mu02||Recombinant Milk Fat Globule EGF Factor 8 (MFGE8)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB286Mu01||Recombinant Milk Fat Globule EGF Factor 8 (MFGE8)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB286Mu01||Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8)||WB; IHC; ICC; IP.|
|PAB286Mu02||Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8)||WB; IHC; ICC; IP.|
|SEB286Mu||ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)||Enzyme-linked immunosorbent assay for Antigen Detection.|