ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)

Cfos; C-Fos; G0S7; Proto-oncogene c-Fos; G0/G1 Switch Regulatory Protein 7; Cellular oncogene fos

Specificity

This assay has high sensitivity and excellent specificity for detection of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS).
No significant cross-reactivity or interference between V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular & Cellular Proteomics A Protein Epitope Signature Tag (PrEST) Library Allows SILAC-based Absolute Quantification and Multiplexed Determination of Protein Copy Numbers in Cell Lines Mcponline: 009613
Molecular and Cellular Biochemistry Geraniol modulates cell proliferation, apoptosis, inflammation, and angiogenesis during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis PubMed: 22729742
African Journal of Traditional, Complementary and Alternative medicines 10(1): 102–112. Proapoptotic, Anti-Cell Proliferative, Anti-Inflammatory and Anti-Angiogenic Potential of Carnosic Acid during 7,12 Dimethylbenz[a]Anthracene-Induced Hamster Buccal Pouch Carcinogenesis PubMed: PMC3746363
Neuropharmacology Levo-corydalmine alleviates vincristine-induced neuropathic pain in mice by inhibiting an NF-kappa B-dependent CXCL1/CXCR2 signaling pathway Pubmed:29518397
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB291Hu01 Recombinant V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) Positive Control; Immunogen; SDS-PAGE; WB.
PAB291Hu01 Polyclonal Antibody to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) WB; IHC; ICC; IP.
MAB291Hu22 Monoclonal Antibody to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) WB; IHC; ICC; IP.
SEB291Hu ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB291Hu Multiplex Assay Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.